PEBP1 antibody (C-Term)

Catalog No. ABIN374198

The Goat Polyclonal anti-PEBP1 antibody is suitable to detect PEBP1 in samples from Human, Mouse, Rat, Cow and Dog. It has been validated for WB, IHC (p) and EIA.

Quick Overview for PEBP1 antibody (C-Term) (ABIN374198)

Target: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This PEBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-PEBP1 Antibody

Binding Specificity: C-Term

Sequence: C-DYVPKLYEQL SGK

Specificity: This antibody detects PBP / RKIP at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog) and Cow.
Species reactivity (tested):Human, Rat.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from C Terminus of the protein sequence according to NP_002558.1 Genename: PEBP1

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/16000. Western blot: 0.01-0.03 μg/mL. Approx 22 kDa band observed in lysates of Human Kidneyfibroblast HEK293, Human lung carcinoma A549 and Human Hepatoblastoma HepG2 celllines (calculated MW of 21.2 kDa according to NP_002558.1). Immunohistochemistry: 1-2 μg/mL. In paraffin embedded Human Brain (Cortex) shows staining near sectect nuclei.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PEBP1

Target: PEBP1 (Phosphatidylethanolamine Binding Protein 1 (PEBP1))

Alternative Name: PEBP1 / RKIP

Background: PBP, also known as RKIP (RAF kinase inhibitor protein), binds ATP, opioids and phosphatidylethanolamine. PBP is able to regulate Aurora kinase B expression and the spindle checkpoint through interaction with the RAF1/MEK/ERK cascade. PBP is able to bind to RAF1 and MEK and competitively disrupt the interaction between these proteins. PBP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. It increases the production of choline acetyltransferase but not acetylcholinesterase. PBP has been implicated as a suppressor of metastasis.Synonyms: HCNPpp, PBP, PEBP, PEBP-1, Phosphatidylethanolamine-binding protein 1, Prostatic-binding protein, Raf kinase inhibitor protein

Gene ID: 5037

NCBI Accession: NP_002558

UniProt: P30086

Pathways: Feeding Behaviour