DBNL antibody (N-Term)

Catalog No. ABIN374214

The Goat Polyclonal anti-DBNL antibody (ABIN374214) specifically detects DBNL in WB. The antibody is reactive with Human, Mouse, Rat and Dog samples.

Quick Overview for DBNL antibody (N-Term) (ABIN374214)

Target: DBNL (Drebrin-Like (DBNL))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This DBNL antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-DBNL Antibody

Binding Specificity: N-Term

Sequence: AANLSRNGPA LQE-C

Specificity: This antibody is expected to recognise all reported isoforms (NP_054782.2, NP_001014436.1, NP_001116428.1).

Cross-Reactivity (Details): Species reactivity (expected):Rat, Canine (Dog).
Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from N Terminus of the protein sequence according to NP_054782.2, NP_001014436.1, NP_001116428.1. Genename: DBNL

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 55 kDa band observed in Jurkat and 3T3 lysates (predictedMW of 45 kDa according to NP_054782).

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DBNL

Target: DBNL (Drebrin-Like (DBNL))

Alternative Name: Drebrin-Like Protein (DBNL)

Background: Cellular morphology, adhesion, and motility occur through dynamic reorganization of actin-based superstructures. Actin-binding proteins are critical for regulating actin polymerization and superstructure formation. The Arp2/3 complex is an actin polymerization-inducing complex that includes Arp2, Arp3, p41-Arc, p34-Arc, p21-Arc, p20 Arc, and p16-Arc. Several actin binding proteins, WASP, cortactin, and Abp1, regulate the activity of the Arp2/3 complex. HIP55 is the mammalian homologue of the yeast HIP55, which has been shown to bind actin and to have roles in endocytosis. HIP55 contains a C-terminal Src homology 3 domain and an N-terminal actin depolymerization factor (ADF-H/C) domain that is homologous to a similar domain found in the actin severing protein, cofilin. HIP55 can bind Src kinases, hematopoietic progenitor kinase 1 (HPK-1), myosin I heavy chain kinase, and dynamin. Thus, HIP55 may be important for regulation of the actin cytoskeleton through its ability to interact with F-actin and various other cytoskeletal and cell signaling proteins. There are three different isoforms.Synonyms: CMAP, Cervical mucin-associated protein, Drebrin-F, HIP-55, HPK1-interacting protein of 55 kDa, SH3 domain-containing protein 7, SH3P7

Molecular Weight: 49kDa (NP_054782).

Gene ID: 28988

NCBI Accession: NP_054782

UniProt: Q9UJU6

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization