PAFAH1B1 antibody (C-Term)
Quick Overview for PAFAH1B1 antibody (C-Term) (ABIN374217)
Target
See all PAFAH1B1 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- C-Term
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Sequence
- TGSVDQTVKV WECR
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Specificity
- This antibody detects PAFAH1B1 / LIS1 at C-term.
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Cross-Reactivity (Details)
- Species reactivity (expected):Human, Rat, Dog, Pig, CowSpecies reactivity (tested):Mouse
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Purification
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
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Immunogen
- Peptide from C Terminus of the protein sequence according to NP_000421.1. Genename: PAFAH1B1
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Application Notes
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Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 45 kDa and 35 kDa bands seen in NIH-3T3 lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Concentration
- 0.5 mg/mL
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Buffer
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Avoid repeated freezing and thawing.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- PAFAH1B1 (Platelet-Activating Factor Acetylhydrolase 1b, Regulatory Subunit 1 (45kDa) (PAFAH1B1))
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Alternative Name
- PAFAH1B1 / LIS1
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Background
- Synonyms: LIS-1, Lissencephaly-1 protein, MDCR, MDS, PAF acetylhydrolase 45 kDa subunit, PAF-AH alpha, PAFAH alpha, PAFAHA, Platelet-activating factor acetylhydrolase IB subunit alpha
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Gene ID
- 5048
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NCBI Accession
- NP_000421
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UniProt
- P43034
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Pathways
- M Phase, Regulation of Cell Size
Target
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