MAP4K1 antibody (N-Term)

Catalog No. ABIN374224

This Goat Polyclonal antibody specifically detects MAP4K1 in WB and EIA. It exhibits reactivity toward Human, Mouse, Rat, Dog, Pig and Cow.

Quick Overview for MAP4K1 antibody (N-Term) (ABIN374224)

Target: MAP4K1 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1))

Reactivity: Human, Mouse, Rat, Dog, Pig, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This MAP4K1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-MAP4K1 Antibody

Binding Specificity: N-Term

Sequence: DVVDPDIFNR DPR-C

Specificity: This antibody is expected to recognise isoform 1 (NP_001036065.1) and isoform 2 (NP_009112.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Pig, Canine (Dog), Cow.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from N Terminus of the protein sequence according to NP_001036065.1, NP_009112.1. Genename: MAP4K1

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.5-2 μg/mL. Approx 95-100 kDa band observed in Jurkat (predicted MW of90 kDa according to NP_009112).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

Target Details for MAP4K1

Target: MAP4K1 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1 (MAP4K1))

Alternative Name: MAP4K1

Background: MAP4K1 or HPKI (hematopoietic progenitor kinase I) is one of these mammalian kinases that have significant sequence similarity to the Saccharomyces Cerevisiae serine/threonine kinase STE20, which relays signals from G protein coupled receptors to cytosolic MAP kinase cascades. MAP4K1 may play a role in the response to environmental stress. It appears to act upstream of the JUN N terminal pathway. It may play a role in hematopoietic lineage decisions and growth regulation.Synonyms: HPK1, Hematopoietic progenitor kinase, MAPK/ERK kinase kinase kinase 1, MEKKK 1, Mitogen-activated protein kinase kinase kinase kinase 1

Gene ID: 11184

NCBI Accession: NP_001036065

UniProt: Q92918

Pathways: TCR Signaling, Signaling of Hepatocyte Growth Factor Receptor