Ubiquitin-Conjugating Enzyme E2C (UBE2C) (C-Term) antibody

Antigen: Ubiquitin-Conjugating Enzyme E2C (UBE2C)

Synonyms: UBCH10, dJ447F3.2, LOC458287

Epitope: C-Term

Clonality: Polyclonal

Host: Goat

Reactivity: Human

Application: Immunoprecipitation (IP), Western Blotting (WB), Enzyme Immunoassay (EIA), Immunofluorescence (IF)

Catalog no.: ABIN374251

Additional Information

Characteristics: Synonyms: Ubiquitin-conjugating enzyme E2 C, Ubiquitin-protein ligase C, Ubiquitin carrier protein C

Alternative name: UBE2C / UBCH10

Gene ID: 11065

Swiss-Prot: O00762

Immunogen: Peptide from C Terminus of the protein sequence according to NP_008950. 1, NP_861515. 1,NP_861516. 1, NP_861517. 1, NP_861518. 1. AA Sequence: C-QETYSKQVTSQEP

Cross-Reactivity: Human

Specificity: This antibody detects UBE2C / UBCH10 at C-term. Species Reactivity: Tested: Human

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 1-3 µg/ml. Approximately 20KDa band was detected in mitotic (i. eNocodazole arrested) HeLa whole cell lysates. Immunoprecipitation: 20KDa band precipitated from mitotic HeLa whole cell lysates usingprotein-G dynabeads. Immunofluorescence: Stained mitotic and late G2 Hela cells, withvery faint or no signal in G1 and S phase cells (fixed using paraformaldehyde, primaryconcentration 2.5 µg/ml, using FITC anti-Goat). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Concentration: 0.5 mg/ml

Purification: Aff - Purified

Buffer: Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin

Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing. Shelf life: one year from despatch.

Restrictions: For Research Use only