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Interferon Regulatory Factor 8 (IRF8) (C-Term) antibody

Details for Product No. ABIN374256
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Antigen
Synonyms zgc:92252, IRF8, MGC140338, DKFZp469B0320, H-ICSBP, ICSBP, ICSBP1, IRF-8, icsbp1, AI893568, Icsbp1, Myls
Epitope
C-Term
(23), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)
(48), (26), (18), (3), (3), (2), (2), (2)
Host
Goat
(46), (7), (1)
Clonality
Polyclonal
Application
Western Blotting (WB)
(50), (23), (9), (8), (6), (5), (1)
Pubmed 1 reference available
Quantity 0.1 mg
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Catalog No. ABIN374256
357.50 $
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Immunogen Peptide from C Terminus of the protein sequence according to NP_002154.1 Genename: IRF8
Sequence C-QRSFFRENQQITV
Specificity This antibody detects IRF8 / ICSBP1 at C-term. Species Reactivity: Tested: Human Expected from sequence similarity: Mouse, Rat, Cow
Purification Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Alternative Name IRF8 / ICSBP1
Background Interferon regulatory factor 8 (IRF8) / Interferon consensus sequence binding protein (ICSBP) is a transcription factor of the interferon (IFN) regulatory factor (IRF) family. Proteins of this family are composed of a conserved DNA binding domain in the N terminal region and a divergent C terminal region that serves as the regulatory domain. The IRF family proteins bind to the IFN stimulated response element (ISRE) and regulate expression of genes stimulated by type I IFNs, namely IFN alpha and IFN beta. IRF family proteins also control expression of IFN alpha and IFN beta regulated genes that are induced by viral infection. IRF8 specifically binds to the upstream regulatory region of type I IFN and IFN inducible MHC class I genes (the interferon consensus sequence (ICS)). Unlike IRF3, IRF8 appears to act as a negative regulator of IFN induced genes in most cases, but IRF8 mediates activation of NF kappaB by the toll like receptor 9 (TLR9) after stimulation by unmethylated CpG DNA in dendritic cells. Finally, it has been shown that IRF8 decreases bcl 2 expression and thus may play a role in chronic myelogenous leukemia.
Alternate names: IRF-8, Interferon consensus sequence-binding protein, Interferon regulatory factor 8
Gene ID 3394
NCBI Accession NP_002154.1
UniProt Q02556
Application Notes Peptide ELISA: Limit dilution 1: 8000. Western blot: 0.5-2 µg/ml. Approx 45-50kDa band seen in K562 lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Tris buffered saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
Expiry Date 12 months
Supplier Images
anti-Interferon Regulatory Factor 8 (IRF8) (C-Term) antibody anti-Interferon Regulatory Factor 8 (IRF8) (C-Term) antibody
Background publications Weisz, Marx, Sharf et al.: "Human interferon consensus sequence binding protein is a negative regulator of enhancer elements common to interferon-inducible genes." in: The Journal of biological chemistry, Vol. 267, Issue 35, pp. 25589-96, 1993 (PubMed).

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