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Ubiquitin-Conjugating Enzyme E2I (UBE2I) (N-Term) antibody

Details for Product No. ABIN374291, Supplier: Log in to see
Antigen
  • UBC9
  • T13J8.70
  • T13J8_70
  • UBIQUITIN-PROTEIN LIGASE
  • ubiquitin conjugating enzyme 9
  • C358B7.1
  • P18
  • 5830467E05Rik
  • F830028O17Rik
  • Ubce2i
  • Ubce9
  • UbcE2A
  • ubc9
  • ube2ia
  • zUbc9
  • ubce9
Epitope
N-Term
35
5
3
2
2
2
1
1
1
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
106
32
25
8
6
3
3
2
2
2
1
1
Host
Goat
70
20
17
1
Clonality
Polyclonal
Conjugate
Un-conjugated
8
8
8
4
4
4
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
96
60
50
9
9
9
6
5
4
2
1
1
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Immunogen Peptide from the N Terminus of the protein sequence according to NP_003336.1, NP_919235.1, NP_919236.1, NP_919237.1 Genename: UBE2I
Sequence SGIALSRLAQ ERK-C
Specificity This antibody detects UBC9 / UBE2I at N-term. It is expected to recognize all reported isoforms (NP_003336.1, NP_919235.1, NP_919236.1 and NP_919237.1).
Cross-Reactivity (Details) Species reactivity (expected):Canien (Dog).
Species reactivity (tested):Human, Mouse, Rat.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Alternative Name UBC9 / UBE2I (UBE2I Antibody Abstract)
Background Synonyms: SUMO-conjugating enzyme UBC9, SUMO-protein ligase, UBCE9, Ubce2i, Ube2i, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I
Gene ID 7329
NCBI Accession NP_003336
UniProt P63279
Pathways
Application Notes Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
 image for anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (N-Term) antibody (ABIN374291) anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (N-Term) antibody
 image for anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (N-Term) antibody (ABIN374291) anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (N-Term) antibody (Image 2)
Background publications Subramaniam, Mealer, Sixt et al.: "Rhes, a physiologic regulator of sumoylation, enhances cross-sumoylation between the basic sumoylation enzymes E1 and Ubc9." in: The Journal of biological chemistry, Vol. 285, Issue 27, pp. 20428-32, 2010 (PubMed).

Ihara, Stein, Schultz: "UBE2I (UBC9), a SUMO-conjugating enzyme, localizes to nuclear speckles and stimulates transcription in mouse oocytes." in: Biology of reproduction, Vol. 79, Issue 5, pp. 906-13, 2008 (PubMed).

Wang, Qiu, Seufert et al.: "Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9." in: The Journal of biological chemistry, Vol. 271, Issue 40, pp. 24811-6, 1996 (PubMed).