Ubiquitin-Conjugating Enzyme E2I (UBE2I) (N-Term) antibody

Details for Product No. ABIN374291
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Antigen
Synonyms P18, UBC9, C358B7.1, ubc9, ube2ia, zUbc9, Ubce9, Ubce2i, 5830467E05Rik, F830028O17Rik, UbcE2A, ubce9, T13J8.70, T13J8_70, UBIQUITIN-PROTEIN LIGASE, ubiquitin conjugating enzyme 9
Epitope
N-Term
(11), (2), (2), (1), (1), (1), (1)
Reactivity
Dog (Canine), Human, Mouse (Murine), Rat (Rattus)
(44), (17), (15), (4), (4), (3), (3), (1), (1), (1)
Host
Goat
(30), (10), (8), (1)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2)
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(47), (27), (19), (9), (9), (4), (3), (2), (1), (1), (1), (1)
Pubmed 3 references available
Catalog no. ABIN374291
Quantity 0.1 mg
Price
357.50 $   Plus shipping costs $45.00
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  • +1 404 474 4654
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Immunogen Peptide from the N Terminus of the protein sequence according to NP_003336.1, NP_919235.1, NP_919236.1, NP_919237.1 Genename: UBE2I
Sequence SGIALSRLAQERK-C
Specificity This antibody detects UBC9 / UBE2I at N-term. It is expected to recognize all reported isoforms (NP_003336.1, NP_919235.1, NP_919236.1 and NP_919237.1). Species Reactivity: Tested: Human, Mouse, Rat. Expected from sequence similarity: Canien (Dog).
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Alternative Name UBC9 / UBE2I
Background Alternate names: SUMO-conjugating enzyme UBC9, SUMO-protein ligase, UBCE9, Ubce2i, Ube2i, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin- protein ligase I
Gene ID 7329
NCBI Accession NP_003336.1
UniProt P63279
Application Notes Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Tris buffered saline, pH~7.3 0.02% Sodium Azide 0.5% BSA
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Supplier Images
anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (N-Term) antibody anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (N-Term) antibody
anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (N-Term) antibody (2) anti-Ubiquitin-Conjugating Enzyme E2I (UBE2I) (N-Term) antibody (Image 2)
Background publications Wang, Qiu, Seufert et al.: "Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9." in: The Journal of biological chemistry, Vol. 271, Issue 40, pp. 24811-6, 1996 (PubMed).

Ihara, Stein, Schultz: "UBE2I (UBC9), a SUMO-conjugating enzyme, localizes to nuclear speckles and stimulates transcription in mouse oocytes." in: Biology of reproduction, Vol. 79, Issue 5, pp. 906-13, 2008 (PubMed).

Subramaniam, Mealer, Sixt et al.: "Rhes, a physiologic regulator of sumoylation, enhances cross-sumoylation between the basic sumoylation enzymes E1 and Ubc9." in: The Journal of biological chemistry, Vol. 285, Issue 27, pp. 20428-32, 2010 (PubMed).

Hosts (30), (10), (8), (1)
Reactivities (44), (17), (15), (4), (4), (3), (3), (1), (1), (1)
Applications (47), (27), (19), (9), (9), (4), (3), (2), (1), (1), (1), (1)
Conjugates (2), (2), (2)
Epitopes (11), (2), (2), (1), (1), (1), (1)
Request Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Order hotline:

  • +1 404 474 4654
  • +1 888 205 9894 (TF)
Validation Images
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