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Ubiquitin-Conjugating Enzyme E2T (Putative) (Ube2t) (C-Term) antibody

Details for Product No. ABIN374292, Supplier: Login to see
Antigen
  • UBE2T
  • wu:fj86h11
  • zgc:153687
  • HR6B
  • PIG50
  • 2700084L22Rik
  • C80607
  • RGD1310816
  • hspc150
Epitope
C-Term
30
15
6
6
5
3
2
2
1
1
Reactivity
Human
67
12
6
Host
Goat
43
15
9
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
3
3
3
Application
Western Blotting (WB)
61
40
9
8
2
1
1
Supplier
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Immunogen Peptide from C Terminus of the protein sequence according to NP_054895.1. Genename: UBE2T
Sequence C-QLVGIEKKFH PDV
Specificity This antibody detects UBE2T at C-term.
Cross-Reactivity (Details) Species reactivity (tested):Human
Purification Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Alternative Name UBE2T (Ube2t Antibody Abstract)
Background Synonyms: HSPC150, Ubiquitin carrier protein T, Ubiquitin-conjugating enzyme E2 T, Ubiquitin-protein ligase T
Gene ID 29089
NCBI Accession NP_054895
UniProt Q9NPD8
Pathways
Application Notes Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.1-0.3 μg/mL. Approx.22 kDa band observed in lysates of cell lines K562,HEK293 and A549 (calculated MW of 22.2 kDa according to NP_054895.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
Supplier Images
 image for anti-Ubiquitin-Conjugating Enzyme E2T (Putative) (Ube2t) (C-Term) antibody (ABIN374292) anti-Ubiquitin-Conjugating Enzyme E2T (Putative) (Ube2t) (C-Term) antibody
Background publications Machida, Machida, Chen et al.: "UBE2T is the E2 in the Fanconi anemia pathway and undergoes negative autoregulation." in: Molecular cell, Vol. 23, Issue 4, pp. 589-96, 2006 (PubMed).