Ubiquitin-Conjugating Enzyme E2T (Putative) (Ube2t) (C-Term) antibody

Details for Product No. ABIN374292
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Antigen
Synonyms UBE2T, wu:fj86h11, zgc:153687, HR6B, PIG50, 2700084L22Rik, C80607, RGD1310816, hspc150
Epitope
C-Term
(9), (8), (7), (6), (5), (4), (1), (1)
Reactivity
Human
(47), (12), (3)
Host
Goat
(29), (14), (4)
Clonality
Polyclonal
Conjugate
Un-conjugated
(2), (2), (2), (2), (2), (2)
Application
Western Blotting (WB)
(46), (29), (7), (4), (2), (1)
Pubmed 1 reference available
Quantity 0.1 mg
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Catalog No. ABIN374292
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Immunogen Peptide from C Terminus of the protein sequence according to NP_054895.1. Genename: UBE2T
Sequence C-QLVGIEKKFH PDV
Specificity This antibody detects UBE2T at C-term. Species Reactivity: Tested: Human
Purification Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
Alternative Name UBE2T
Background Alternate names: HSPC150, Ubiquitin carrier protein T, Ubiquitin-conjugating enzyme E2 T, Ubiquitin-protein ligase T
Gene ID 29089
NCBI Accession NP_054895
UniProt Q9NPD8
Application Notes Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.1-0.3 µg/ml. Approx.22kDa band observed in lysates of cell lines K562,HEK293 and A549 (calculated MW of 22.5kDa according to NP_054895.1). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.5 mg/mL
Buffer Tris buffered saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
Expiry Date 12 months
Supplier Images
anti-Ubiquitin-Conjugating Enzyme E2T (Putative) (Ube2t) (C-Term) antibody anti-Ubiquitin-Conjugating Enzyme E2T (Putative) (Ube2t) (C-Term) antibody
Background publications Machida, Machida, Chen et al.: "UBE2T is the E2 in the Fanconi anemia pathway and undergoes negative autoregulation." in: Molecular cell, Vol. 23, Issue 4, pp. 589-96, 2006 (PubMed).

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