FOXC1 antibody (C-Term)

Catalog No. ABIN374336

There are 3+ publications for this product available. The Goat Polyclonal anti-FOXC1 antibody is suitable to detect FOXC1 in samples from Human, Mouse and Zebrafish (Danio rerio). It has been validated for WB, IHC (p), ChIP and EIA.

Quick Overview for FOXC1 antibody (C-Term) (ABIN374336)

Target: FOXC1 (Forkhead Box C1 (FOXC1))

Reactivity: Human, Mouse, Zebrafish (Danio rerio)

Host: Goat

Clonality: Polyclonal

Conjugate: This FOXC1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Chromatin Immunoprecipitation (ChIP), Enzyme Immunoassay (EIA)

Product Details anti-FOXC1 Antibody

Binding Specificity: C-Term

Sequence: RTSGAFVYDC SKF

Specificity: This antibody detects FOXC1 / FKHL7 / FREAC3 at C-term.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse, Zebrafish.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C-Terminus of the protein sequence according to NP_001444.2. Genename: FOXC1

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.5-1.5 μg/mL. Approx 55 kDa band observed in Human Bone Marrow lysates(calculated MW of 56.5 kDa according to NP_001444.1). Immunohistochemistry: 3 μg/mL. In paraffin embedded Human Kidney shows nuclearstaining of some cells in the glomeruli.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.

References for anti-FOXC1 antibody (ABIN374336)

Doucette, Footz, Walter: "FOXC1 Regulates Expression of Prostaglandin Receptors Leading to an Attenuated Response to Latanoprost." in: Investigative ophthalmology & visual science, Vol. 59, Issue 6, pp. 2548-2554, (2019) (PubMed).

Fatima, Wang, Uchida, Norden, Liu, Culver, Dietz, Culver, Millay, Mukouyama, Kume: "Foxc1 and Foxc2 deletion causes abnormal lymphangiogenesis and correlates with ERK hyperactivation." in: The Journal of clinical investigation, Vol. 126, Issue 7, pp. 2437-51, (2017) (PubMed).

Ito, Goping, Berry, Walter: "Dysfunction of the stress-responsive FOXC1 transcription factor contributes to the earlier-onset glaucoma observed in Axenfeld-Rieger syndrome patients." in: Cell death & disease, Vol. 5, pp. e1069, (2014) (PubMed).

Target Details for FOXC1

Target: FOXC1 (Forkhead Box C1 (FOXC1))

Alternative Name: FOXC1 / FKHL7 / FREAC3

Background: FOXC1 belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. The specific function of this gene has not yet been determined, however, it has been shown to play a role in the regulation of embryonic and ocular development. Mutations in this gene cause various glaucoma phenotypes including primary congenital glaucoma, autosomal dominant iridogoniodysgenesis anomaly, and Axenfeld-Rieger anomaly.Synonyms: FREAC-3, Forkhead box protein C1, Forkhead-related protein FKHL7, Forkhead-related transcription factor 3

Molecular Weight: 56.8kDa (NP_001444.1).

Gene ID: 2296

NCBI Accession: NP_001444

UniProt: Q12948

Pathways: Chromatin Binding, Glycosaminoglycan Metabolic Process