FOXC2 antibody (C-Term)

Catalog No. ABIN374337

The Goat Polyclonal anti-FOXC2 antibody (ABIN374337) specifically detects FOXC2 in WB and IHC (p). The antibody is reactive with Human, Mouse, Rat, Cow and Dog samples.

Quick Overview for FOXC2 antibody (C-Term) (ABIN374337)

Target: FOXC2 (Forkhead Box C2 (MFH-1, Mesenchyme Forkhead 1) (FOXC2))

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This FOXC2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-FOXC2 Antibody

Binding Specificity: C-Term

Sequence: RHAAPYSYDC TKY

Specificity: This antibody detects FOXC2 / FKHL14 / MFH1 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from C Terminus of the protein sequence according to NP_005242.1 Genename: FOXC2

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 8000. Western blot: 1-3 μg/mL. Approx 50-55 kDa band observed in Human Breast Cancer lysates(calculated MW of 53.5 kDa according to NP_005242.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FOXC2

Target: FOXC2 (Forkhead Box C2 (MFH-1, Mesenchyme Forkhead 1) (FOXC2))

Alternative Name: FOXC2 / FKHL14 / MFH1

Background: FOXC2 belongs to the forkhead family of transcription factors which is characterized by a distinct DNA binding forkhead domain. The specific function of this gene has not yet been determined, however, it may play a role in the development of mesenchymal tissues.Synonyms: FKH-14, Forkhead box protein C2, Forkhead-related protein FKHL14, MFH-1, Mesenchyme fork head protein 1, Transcription factor FKH-14

Gene ID: 2303

NCBI Accession: NP_005242

UniProt: Q99958