Anillin antibody (C-Term)

Catalog No. ABIN374360

The Goat Polyclonal anti-Anillin antibody is suitable to detect Anillin in samples from Human, Mouse and Cow. It has been validated for WB, IHC (p) and EIA.

Quick Overview for Anillin antibody (C-Term) (ABIN374360)

Target: Anillin (ANLN) (Anillin, Actin Binding Protein (ANLN))

Reactivity: Human, Mouse, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This Anillin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Anillin Antibody

Binding Specificity: C-Term

Sequence: WQPDACYKPI GKP

Specificity: This antibody detects Anillin / ANLN at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Cow.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_061155.2. Genename: ANLN

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 64000. Western blot: Experiments gave an approx 75 kDa band in Mouse Brain lysates after 0.3 μg/mL antibody staining. It was successfully blocked by incubation with the immunizingpeptide. Immunohistochemistry: 3-10 μg/mL. In paraffin embedded Human Kidney shows staining ofnuclei in some cells of renal tubules.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for Anillin

Target: Anillin (ANLN) (Anillin, Actin Binding Protein (ANLN))

Alternative Name: Anillin / ANLN

Background: Anillin is required for cytokinesis. It is essential for the structural integrity of the cleavage furrow and for completion of cleavage furrow ingression.Synonyms: Actin-binding protein anillin

Gene ID: 54443

NCBI Accession: NP_061155

UniProt: Q9NQW6