ATP6AP2 antibody (C-Term)

Catalog No. ABIN374363

There are 2+ publications for this product available. The Goat Polyclonal anti-ATP6AP2 antibody is suitable to detect ATP6AP2 in samples from Human, Rat, Mouse and Cow. It has been validated for WB, IHC (p) and EIA.

Quick Overview for ATP6AP2 antibody (C-Term) (ABIN374363)

Target: ATP6AP2 (ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2))

Reactivity: Human, Rat, Mouse, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This ATP6AP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-ATP6AP2 Antibody

Binding Specificity: C-Term

Sequence: C-SIIYRMTNQK IRMD

Specificity: This antibody detects Renin Receptor at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Cow.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_005756.2 Genename: ATP6AP2

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.25-1 μg/mL. Approx 37 kDa band observed in Human Kidney lysates(predicted size of approx.40Kda according to NP_005756).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-ATP6AP2 antibody (ABIN374363)

Happold, Roth, Wick, Schmidt, Florea, Silginer, Reifenberger, Weller: "Distinct molecular mechanisms of acquired resistance to temozolomide in glioblastoma cells." in: Journal of neurochemistry, Vol. 122, Issue 2, pp. 444-55, (2013) (PubMed).

Zhu, Yoneda, Demura, Karashima, Usukura, Yamagishi, Takeda: "Effect of mineralocorticoid receptor blockade on the renal renin-angiotensin system in Dahl salt-sensitive hypertensive rats." in: Journal of hypertension, Vol. 27, Issue 4, pp. 800-5, (2009) (PubMed).

Target Details for ATP6AP2

Target: ATP6AP2 (ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2))

Alternative Name: Renin Receptor

Background: Synonyms: ATP6AP2, ATP6IP2, ATP6M8-9, ATPase H(+)-transporting lysosomal accessory protein 2, ATPase H(+)-transporting lysosomal-interacting protein 2, CAPER, ELDF10, ER-localized type I transmembrane adaptor, Embryonic liver differentiation factor 10, HT028, MSTP009, N14F, PSEC0072, Renin/prorenin receptor, Vacuolar ATP synthase membrane sector-associated protein M8-9

Gene ID: 10159

NCBI Accession: NP_005756

UniProt: O75787

Pathways: ACE Inhibitor Pathway, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones