MPP6 antibody (N-Term)

Catalog No. ABIN374387

The Goat Polyclonal anti-MPP6 antibody is suitable to detect MPP6 in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for MPP6 antibody (N-Term) (ABIN374387)

Target: MPP6 (Membrane Protein, Palmitoylated 6 (MAGUK P55 Subfamily Member 6) (MPP6))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This MPP6 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MPP6 Antibody

Binding Specificity: N-Term

Sequence: QQVLENLTEL PSS-C

Specificity: This antibody detects MAGUK p55 subfamily member 6 at N-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Dog, Pig, Cow.
Species reactivity (tested):Human.

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from N Terminus of the protein sequence according to NP_057531.2 Genename: MPP6

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 128000. Western blot: 0.3-1 μg/mL. Approx 60 kDa band observed in lysates of cell line HEK293(calculated MW of 61.0 kDa according to NP_057531.2). An additional band of unknownidentity was also consistently observed at 20 kDa. This band was successfully blocked byincubation with the immunising peptide. We would appreciate any feedback from peoplein the field - have any such results been reported with other antibodies/lysates? Have anyfurther splice variants/modified forms been reported?
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for MPP6

Target: MPP6 (Membrane Protein, Palmitoylated 6 (MAGUK P55 Subfamily Member 6) (MPP6))

Alternative Name: MPP6

Background: Progression of cells from interphase to mitosis involves alterations in cell structures and activities. The transition from G2 to M phase is induced by M phase promoting factor, or MPF. In M phase, many proteins are phosphorylated directly by MPF or indirectly by kinases activated by MPF. These M phase phosphoproteins (MPPs, or MPHOSPHs) permit disassembly of interphase structures and generation of M phase enzymatic activities and structures. VAM1 or MPP6 is thought to be a nucleolus specific exosome co factor, required for its role in the maturation of 5.8S rRNA.Synonyms: MAGUK p55 subfamily member 6, VAM1, Veli-associated MAGUK 1

Gene ID: 51678

NCBI Accession: NP_057531

UniProt: Q9NZW5

Pathways: Nucleotide Phosphorylation