VPS29 antibody (C-Term)

Catalog No. ABIN374400

The Goat Polyclonal anti-VPS29 antibody (ABIN374400) specifically detects VPS29 in WB. The antibody is reactive with Human, Mouse, Rat, Dog and Cow samples.

Quick Overview for VPS29 antibody (C-Term) (ABIN374400)

Target: VPS29 (Vacuolar Protein Sorting 29 (VPS29))

Reactivity: Human, Mouse, Rat, Dog, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This VPS29 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-VPS29 Antibody

Binding Specificity: C-Term

Sequence: C-DDVKVERIEY KKP

Specificity: This antibody detects VPS29 at C-term. It is expected to recognise both Human isoforms.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog) and Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_057310.1 and NP_476528.1 Genename: VPS29

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/16000. Western blot: 0.1-0.3 μg/mL. Approx 20+21 kDa bands observed in Human Spleen lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 with 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for VPS29

Target: VPS29 (Vacuolar Protein Sorting 29 (VPS29))

Alternative Name: VPS29

Background: VPS29, VPS30, and VPS35 proteins are required for the normal recycling of Vps10p, a late Golgi transmembrane protein that acts as the sorting receptor for soluble vacuolar hydrolases, from the prevacuolar endosome back to the Golgi.Synonyms: DC15, DC7, MDS007, PEP11, Vacuolar protein sorting-associated protein 29, Vesicle protein sorting 29

Molecular Weight: 20.5kDa (NP_057310.1) and 20.9kDa (NP_476528.1).

Gene ID: 51699

NCBI Accession: NP_057310

UniProt: Q9UBQ0