VPS35 antibody (C-Term)

Catalog No. ABIN374403

The Goat Polyclonal anti-VPS35 antibody (ABIN374403) specifically detects VPS35 in WB, IF, IHC (p) and EIA. The antibody is reactive with Human, Mouse, Rat and Cow samples.

Quick Overview for VPS35 antibody (C-Term) (ABIN374403)

Target: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Reactivity: Human, Mouse, Rat, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This VPS35 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-VPS35 Antibody

Binding Specificity: C-Term

Sequence: C-SPESEGPIYE GLIL

Specificity: This antibody detects VPS35 / MEM3 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Syntehtic peptide from the C-Terminus of Human VPS35 (NP_060676.2). Genename: VPS35

Application Details

Application Notes: Peptide ELISA: 1/16000. Western blot: Approx 90 kDa band observed in Human Brain and Testis lysates (predictedMW of 90 kDa according to NP_060676.2). Recommended concentration: 1 - 3 μg/mL. Immunohistochemistry on paraffin sections: Human Testis, shows positive staining inLeydig cells, and cytoplasmic staining (dot-like) of some epithelial cells of the epididymisand of the rete testis. Immunoflurescence: Staining of VPS35 (red) in human melanoma cells 1205LU: Please seeScott KL et al. , Nature.2009 Jun 25,459(7250): 1085-90. , PMID: 19553991.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for VPS35

Target: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Alternative Name: VPS35 / MEM3

Background: Synonyms: Maternal-embryonic 3, TCCCTA00141, Vacuolar protein sorting-associated protein 35, Vesicle protein sorting 35

Gene ID: 55737

NCBI Accession: NP_060676

UniProt: Q96QK1