PITPNM1 antibody (C-Term)

Catalog No. ABIN374422

This Goat Polyclonal antibody specifically detects PITPNM1 in WB and IF. It exhibits reactivity toward Human, Mouse, Cow and Dog.

Quick Overview for PITPNM1 antibody (C-Term) (ABIN374422)

Target: PITPNM1 (Phosphatidylinositol Transfer Protein, Membrane-Associated 1 (PITPNM1))

Reactivity: Human, Mouse, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This PITPNM1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-PITPNM1 Antibody

Binding Specificity: C-Term

Sequence: C-KARSISLKLD SEE

Specificity: This antibody detects NIR2 at C-term and is expected to recognise both reported isoforms.

Cross-Reactivity (Details): Species reactivity (expected):Human, Dog, Cow.
Species reactivity (tested):Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the C-Terminus of the protein sequence according to NP_004901.2, NP_001124320.1. Genename: PITPNM1

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 8000. Western blot: Customer's experiments gave a band at approx 160 kDa in lysates of a MouseMacrophage cell line after 2 μg/mL antibody staining (calculated MW of 130 kDa accordingto NP_004901.1). In ICC the customer found staining of vesicular cytoplasm in the samecell line.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PITPNM1

Target: PITPNM1 (Phosphatidylinositol Transfer Protein, Membrane-Associated 1 (PITPNM1))

Alternative Name: NIR2

Background: NIR2 belongs to a family of proteins that share homology with the Drosophila retinal degeneration B (rdgB) protein. Nir2, a human homolog of Drosophila melanogaster retinal degeneration B protein, is essential for cytokinesis. A specific Thr residue in the Nir2 PI-transfer domain provides a regulatory site for targeting to lipid droplets. This may affect intracellular lipid trafficking and distribution & explain the dominant effect of the RdgB-T59E mutant on retinal degeneration.Synonyms: DRES9, Membrane-associated phosphatidylinositol transfer protein 1, NIR2, PITPNM, PITPNM1

Gene ID: 9600

NCBI Accession: NP_004901

UniProt: O00562