BAF53A antibody (C-Term)

Catalog No. ABIN374427

The Goat Polyclonal anti-BAF53A antibody (ABIN374427) specifically detects BAF53A in WB. The antibody is reactive with Human, Mouse, Rat, Cow and Dog samples.

Quick Overview for BAF53A antibody (C-Term) (ABIN374427)

Target: BAF53A (Actin-Like 6A (BAF53A))

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This BAF53A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BAF53A Antibody

Binding Specificity: C-Term

Sequence: YEEGGKQCVE RKCP

Specificity: This antibody detects ACTL6A / BAF53 at C-term.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Dog, CowSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from C Terminus of the protein sequence according to NP_004292.1, NP_817126.1, NP_057272.1. Genename: ACTL6A / ACTL6B

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 64000. Western Blot: 1-3 μg/mL. Approx 48 kDa band observed in Hela lysates (predicted MW of48 kDa according to NP_057272, NP_004292).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.

Target Details for BAF53A

Target: BAF53A (Actin-Like 6A (BAF53A))

Alternative Name: ACTL6A / BAF53

Background: ACTL6A is a family member of actin-related proteins (ARPs), which share significant amino acid sequence identity to conventional actins. Both actins and ARPs have an actin fold, which is an ATP-binding cleft, as a common feature. The ARPs are involved in diverse cellular processes, including vesicular transport, spindle orientation, nuclear migration and chromatin remodeling. ACTL6A is a 53 kDa subunit of the BAF (BRG1/brm-associated factor) complex in mammals, which is functionally related to SWI/SNF complex in S. cerevisiae and Drosophila, the latter is thought to facilitate transcriptional activation of specific genes by antagonizing chromatin-mediated transcriptional repression. Together with beta-actin, it is required for maximal ATPase activity of BRG1, and for the association of the BAF complex with chromatin/matrix. It is required for maximal ATPase activity of SMARCA4/BRG1 and for association of the SMARCA4/BRG1 containing remodelling complex BAF with chromatin/nuclear matrix. ACTL6A is a component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histone H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when recruited to sites of DNA damage.Synonyms: 53 kDa BRG1-associated factor A, Actin-like protein 6A, Actin-related protein Baf53a, ArpNbeta, BAF53A, INO80 complex subunit K, INO80K

Gene ID: 86

NCBI Accession: NP_004292

UniProt: O96019

Pathways: Chromatin Binding, Photoperiodism