LIME antibody (C-Term)

Catalog No. ABIN374438

The Goat Polyclonal anti-LIME antibody is suitable to detect LIME in samples from Mouse and Rat. It has been validated for WB.

Quick Overview for LIME antibody (C-Term) (ABIN374438)

Target: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Reactivity: Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This LIME antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-LIME Antibody

Binding Specificity: C-Term

Sequence: C-ENVYESIKEM GL

Specificity: This antibody recognizes LIME1.

Cross-Reactivity (Details): Species reactivity (expected):Rat.
Species reactivity (tested):Mouse

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the C Terminus of the protein sequence according to NP_076173.1.

Application Details

Application Notes: Peptide ELISA: 1/128000. Western Blot: 0.5-2.0 μg/mL, Approx 32 kDa band observed in Mouse Heart lysates(predicted MW of 32 kDa according to NP_076173.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for LIME

Target: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Alternative Name: LIME1

Background: LIME (Lck-interacting molecule) is a new raft-associated adaptor protein involved in regulation of T cell activation by coreceptors. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation.Synonyms: LIME, LIME-1, LIME1, Lck-interacting membrane protein, Lck-interacting molecule, Lck-interacting transmembrane adapter 1

Gene ID: 54923

NCBI Accession: NP_060276

UniProt: Q9H400