PPARD antibody (C-Term, Isoform 1)

Catalog No. ABIN374465

The Goat Polyclonal anti-PPARD antibody is suitable to detect PPARD in samples from Human, Mouse, Rat, Cow and Pig. It has been validated for WB.

Quick Overview for PPARD antibody (C-Term, Isoform 1) (ABIN374465)

Target: PPARD (Peroxisome Proliferator-Activated Receptor delta (PPARD))

Reactivity: Human, Mouse, Rat, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This PPARD antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PPARD Antibody

Binding Specificity: C-Term, Isoform 1

Sequence: CHPLLQEIYK DMY

Specificity: This antibody is expected to recognize isoform 1 (NP_006229) of PPAR delta, but not isoform 2, a shorter protein (NP_803184) with a different C-terminus.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Porcine, Bovine.
Species reactivity (tested):Human, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_006229.1. Genename: PPARD

Application Details

Application Notes: Peptide ELISA: 1/16000. Western blot: 0.3 - 1 μg/mL
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PPARD

Target: PPARD (Peroxisome Proliferator-Activated Receptor delta (PPARD))

Alternative Name: PPAR-delta

Background: PPARD is a member of the nuclear hormone receptor subfamily. Upon activation, PPARD forms heterodimers with retinoid X receptors (RXRs), and functions to regulate transcription of specific gene sequences. PPARD controls genes involved in lipid metabolism, and enhances fatty acid catabolism and energy uncoupling in adipose tissue and skeletal muscle. PPARD has also been implicated in the proliferation and differentiation of preadipocytes, enterocytes, keratinocytes and the placenta. PPARD agonists could be used therapeutically to target multiple components of the metabolic syndrome, including insulin resistance, hyperglycemia, dyslipidemia, and atherosclerosis.Synonyms: NR1C2, NUCI, Nuclear hormone receptor 1, Nuclear receptor subfamily 1 group C member 2, PPAR-beta, PPARB, PPARD, Peroxisome proliferator-activated receptor delta

Gene ID: 5467

NCBI Accession: NP_001165289

UniProt: Q03181

Pathways: Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Monocarboxylic Acid Catabolic Process, Smooth Muscle Cell Migration, Positive Regulation of fat Cell Differentiation