ABCC4 antibody (C-Term)

Catalog No. ABIN374484

Cited in 7+ publications. The Goat Polyclonal anti-ABCC4 antibody (ABIN374484) specifically detects ABCC4 in WB, IHC (p), IF and EIA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for ABCC4 antibody (C-Term) (ABIN374484)

Target: ABCC4 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 4 (ABCC4))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This ABCC4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Product Details anti-ABCC4 Antibody

Binding Specificity: C-Term

Sequence: CNGQPSTLTI FETAL

Specificity: This antibody antibody is expected to recognize isoform 1 (NP_005836.2) only.

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C-Terminus of the protein sequence (NP_005836.2). Genename: ABCC4

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/64000. Western blot: 1-3 μg/mL. Approx 170 kDa band observed in rat renal cortex membranes(calculated MW of 140 kDa according to Rat NP_596902.1 and 150 kDa according to HumanNP_005836.2). Immunohistochemistry on Paraffin Sections: 2-4 μg/mL. In paraffin embedded Human Prostate shows strong cytoplasmic staining in small flatbasal cells and weak cytoplasmic staining in the columnar secretory cells of theepithelium.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-ABCC4 antibody (ABIN374484)

Zhang, Dong, Wang, Ma, Wang, Wu: "Stable knock-down of efflux transporters leads to reduced glucuronidation in UGT1A1-overexpressing HeLa cells: the evidence for glucuronidation-transport interplay." in: Molecular pharmaceutics, Vol. 12, Issue 4, pp. 1268-78, (2016) (PubMed).

Li, Sun, Zhang, Wang, Wu: "Efflux transport of chrysin and apigenin sulfates in HEK293 cells overexpressing SULT1A3: The role of multidrug resistance-associated protein 4 (MRP4/ABCC4)." in: Biochemical pharmacology, Vol. 98, Issue 1, pp. 203-14, (2016) (PubMed).

Quan, Wang, Dong, Zhang, Wu: "Characterization of chrysin glucuronidation in UGT1A1-overexpressing HeLa cells: elucidating the transporters responsible for efflux of glucuronide." in: Drug metabolism and disposition: the biological fate of chemicals, Vol. 43, Issue 4, pp. 433-43, (2015) (PubMed).

Gori, Rodriguez, Pellegrini, Achtari, Hornung, Chardonnens, Wunder, Fiche, Canny: "Augmented epithelial multidrug resistance-associated protein 4 expression in peritoneal endometriosis: regulation by lipoxin A(4)." in: Fertility and sterility, Vol. 99, Issue 7, pp. 1965-73.e2, (2013) (PubMed).

Montani, Hermanns, Herrmanns, Müntener, Wild, Sulser, Kristiansen: "Multidrug resistance protein 4 (MRP4) expression in prostate cancer is associated with androgen signaling and decreases with tumor progression." in: Virchows Archiv : an international journal of pathology, Vol. 462, Issue 4, pp. 437-43, (2013) (PubMed).

Brachs, Lang, Buslei, Purohit, Fürnrohr, Kalbacher, Jäck, Mielenz: "Monoclonal antibodies to discriminate the EF hand containing calcium binding adaptor proteins EFhd1 and EFhd2." in: Monoclonal antibodies in immunodiagnosis and immunotherapy, Vol. 32, Issue 4, pp. 237-45, (2013) (PubMed).

Weerachayaphorn, Mennone, Soroka, Harry, Hagey, Kensler, Boyer: "Nuclear factor-E2-related factor 2 is a major determinant of bile acid homeostasis in the liver and intestine." in: American journal of physiology. Gastrointestinal and liver physiology, Vol. 302, Issue 9, pp. G925-36, (2012) (PubMed).

Target Details for ABCC4

Target: ABCC4 (ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 4 (ABCC4))

Alternative Name: ABCC4 / MRP4

Background: ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). MRP4 is a member of the MRP subfamily which is involved in multi-drug resistance. MRP4 may play a role in cellular detoxification as a pump for its substrate, organic anions. Alternative splicing results in multiple splice variants encoding different isoforms.Synonyms: ATP-binding cassette sub-family C member 4, MOAT-B, MRP/cMOAT-related ABC transporter, Multi-specific organic anion transporter B, Multidrug resistance-associated protein 4

Molecular Weight: 149kDa (Rat NP_596902.1) and 150kDa (Human NP_005836.2).

Gene ID: 10257, 9606

UniProt: O15439