RBP1 antibody (C-Term)

Catalog No. ABIN374530

This Goat Polyclonal antibody specifically detects RBP1 in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Mouse and Cow.

Quick Overview for RBP1 antibody (C-Term) (ABIN374530)

Target: RBP1 (Retinol Binding Protein 1, Cellular (RBP1))

Reactivity: Human, Mouse, Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This RBP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-RBP1 Antibody

Binding Specificity: C-Term

Sequence: VEGVVCKQVF KKVQ

Specificity: This antibody recognizes Retinol-binding protein 1 (RBP1).

Cross-Reactivity (Details): Species reactivity (expected):Bovine.
Species reactivity (tested):Human, Mouse.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_002890.1. Genename: RBP1

Application Details

Application Notes: Peptide ELISA: 1/16000. Western blot: Approx 16 kDa band observed in Murine NIH 3T3 lysates (calculated MW of15.6 kDa according to NP_002890.1). Recommended concentration: 0.1 - 0.3 μg/mL. Immunohistochemistry on paraffin sections: Human Pancreas shows vesicular staining inthe acinar cytoplasm but oriented towards the interlobular space. . Recommendedconcentration: 5 - 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for RBP1

Target: RBP1 (Retinol Binding Protein 1, Cellular (RBP1))

Alternative Name: Retinol-Binding Protein 1

Background: RBP1 is a carrier protein involved in the transport of retinol (vitamin A alcohol) from the liver storage site to peripheral tissue. Vitamin A is a fat-soluble vitamin necessary for growth, reproduction, differentiation of epithelial tissues, and vision. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli. Detected in nearly all the tissues with higher expression in adult ovary, pancreas, pituitary gland and adrenal gland, and fetal liver.Synonyms: CRBP-I, CRBP1, Cellular retinol-binding protein, Cellular retinol-binding protein I, RBP1

Gene ID: 5947

NCBI Accession: NP_002890

UniProt: P09455