Catalase antibody (Internal Region)

Catalog No. ABIN374696

The Goat Polyclonal anti-Catalase antibody is suitable to detect Catalase in samples from Human, Mouse and Dog. It has been validated for WB, IHC (p) and EIA.

Quick Overview for Catalase antibody (Internal Region) (ABIN374696)

Target: Catalase (CAT)

Reactivity: Human, Mouse, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This Catalase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-Catalase Antibody

Binding Specificity: Internal Region

Sequence: C-DKYNAEKPKN AIHT

Specificity: This antibody recognizes Catalase.

Cross-Reactivity (Details): Species reactivity (expected):Canine.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Syntehtic Peptide from the internal region of Human Catalase (NP_001743.1). Genename: CAT

Application Details

Application Notes: ELISA: Dtection Limit: 1/32000. Western Blot: 0.3-1 μg/mL. Immunohistochemistry on Paraffin Sections: 2-4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Catalase

Target: Catalase (CAT)

Alternative Name: Catalase

Background: Catalase is the classical marker for peroxisomes and is the most abundant protein within peroxisomes. It is found in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide.Synonyms: CAT, Peroxisome Marker

Molecular Weight: 59.8kDa (NP_001743.1).

Gene ID: 847, 9606

UniProt: P04040

Pathways: Cellular Glucan Metabolic Process, Cell RedoxHomeostasis, Photoperiodism