GRP94 antibody (Internal Region)

Catalog No. ABIN374699

The Goat Polyclonal anti-GRP94 antibody is suitable to detect GRP94 in samples from Human, Mouse, Rat, Cow, Dog, Pig and Zebrafish (Danio rerio). It has been validated for WB, IHC (p) and EIA.

Quick Overview for GRP94 antibody (Internal Region) (ABIN374699)

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig, Zebrafish (Danio rerio)

Host: Goat

Clonality: Polyclonal

Conjugate: This GRP94 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-GRP94 Antibody

Binding Specificity: Internal Region

Sequence: C-KEGVKFDESE KTKE

Specificity: This antibody recognizes GPR94/TRA1 (Internal).

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog), Pig, Cow and Zebrafish.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_003290.1. Genename: HSP90B1

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/32000. Western blot: 0.01-0.03 μg/mL. Approx 100 kDa band observed in lysates of cell lineNIH/3T3 (calculated MW of 92.0 kDa according to NP_003290.1). Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Liver shows staining insome hepatocyte nuclei and weak cytoplasm staining of hepatocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GRP94

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Alternative Name: Endoplasmin / HSP90B1 / TRA1

Background: Glucose regulated protein 94 (GRP 94) is a resident protein of the endoplasmic reticulum (ER) and is induced by the accumulation of unfolded proteins suggesting that it might associate transiently with a variety of newly synthesized secretory and membrane proteins or permanently with mutant or defective proteins. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C terminus of GRP 94 and other resident ER proteins including GRP 78 and protein disulfide isomerase (PDI). The presence of carboxy terminal KDEL appears to be necessary for retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor. GRP 94 is also a low affinity, high capacity calcium binding protein, though it's role, if any, in calcium regulation is not understood.Synonyms: 94 kDa glucose-regulated protein, GRP94, Heat shock protein 90 kDa beta member 1, Tumor rejection antigen 1

Gene ID: 7184

NCBI Accession: NP_003290

UniProt: P14625

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades