Hsc70 antibody (Internal Region, Isoform 1)

Catalog No. ABIN374703

The Goat Polyclonal anti-Hsc70 antibody (ABIN374703) specifically detects Hsc70 in WB and EIA. The antibody is reactive with Human, Rat, Mouse and Dog samples.

Quick Overview for Hsc70 antibody (Internal Region, Isoform 1) (ABIN374703)

Target: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Reactivity: Human, Rat, Mouse, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This Hsc70 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Hsc70 Antibody

Binding Specificity: Internal Region, Isoform 1

Sequence: C-EKLQGKINDE DKQK

Specificity: This antibody is expected to recognise isoform 1 of HSPA8 (NP_006588.1) only.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein sequence

Application Details

Application Notes: ELISA: 1/4000. Western Blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Hsc70

Target: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Alternative Name: HSPA8 / HSC70

Background: The family of approximately 70 kDa heat-shock proteins, HSP70, contains both heat-inducible and constitutively expressed members, the latter of which are sometimes called heat-shock cognate proteins (HSCs). HSPA8 gene encodes Hsc70 protein, a heat-shock cognate protein that binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell.Synonyms: HSP73, HSPA10, Heat shock 70 kDa protein 8, Heat shock cognate 71 kDa protein

Gene ID: 3312

NCBI Accession: NP_006588

UniProt: P11142