Hsc70 antibody (N-Term)

Catalog No. ABIN650754

Cited in 2+ publications. The Rabbit Polyclonal anti-Hsc70 antibody (Clone RB17655) (ABIN650754) specifically detects Hsc70 in WB, IF, FACS and IHC (p). The antibody is reactive with Human samples.

Quick Overview for Hsc70 antibody (N-Term) (ABIN650754)

Target: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Hsc70 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB17655

Product Details anti-Hsc70 Antibody

Binding Specificity: AA 82-110, N-Term

Predicted Reactivity: B, C, Ha, Hs, M, Rat

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This HSPA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-110 amino acids from the N-terminal region of human HSPA8.

Isotype: Ig Fraction

Application Details

Application Notes: IF: 1:10~50. WB: 1:1000. IHC-P: 1:10~50. FC: 1:10~50

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-Hsc70 antibody (ABIN650754)

Fitter, Gronthos, Ooi, Zannettino: "The Mesenchymal Precursor Cell Marker Antibody STRO-1 Binds to Cell Surface Heat Shock Cognate 70." in: Stem cells (Dayton, Ohio), Vol. 35, Issue 4, pp. 940-951, (2017) (PubMed).

Li, Li, Wang, Cui, Gong, Qu, Zhong, Zhou, Zhou, Gao, Li: "Quantitative proteomic study of human prostate cancer cells with different metastatic potentials." in: International journal of oncology, Vol. 48, Issue 4, pp. 1437-46, (2016) (PubMed).

Target Details for Hsc70

Target: Hsc70 (HSPA8) (Heat Shock 70kDa Protein 8 (HSPA8))

Alternative Name: HSPA8

Background: HSPA8 belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. HSPA8 is a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. The protein also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell.

Molecular Weight: 70898

Gene ID: 3312

NCBI Accession: NP_006588, NP_694881

UniProt: P11142