WT1 antibody (N-Term)

Catalog No. ABIN374714

The Goat Polyclonal anti-WT1 antibody has been validated for WB. It is suitable to detect WT1 in samples from Human.

Quick Overview for WT1 antibody (N-Term) (ABIN374714)

Target: WT1 (Wilms Tumor 1 (WT1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This WT1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-WT1 Antibody

Binding Specificity: N-Term

Specificity: This antibody reacts to Wilms tumor 1. This antibody is expected to recognise all four reported isoforms.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence QDPASTCVPEPASQH, from the N Terminus of the protein sequence

Application Details

Application Notes: ELISA: 1/4000. Western Blot: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3, 0.02 % sodium azide, 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Alternative Name: WT1 / Wilms Tumor Protein

Background: WT1 encodes multiple isoforms of a zinc finger transcription factor essential for correct mammalian urogenital development. Different WT1 isoforms are thought to regulate transcription and participate in mRNA processing, functions reflected by a complex sub nuclear distribution. In humans, the preservation of the correct isoform ratio and their localization has been reported to be vital, and its disruption to result in disease. This gene has been reported as a useful marker for several types of illnesses. Mutations in this gene lead to the development of Wilm's Tumor, a sporadic and familial childhood kidney tumor.Synonyms: WT33

Gene ID: 7490, 9606

UniProt: P19544

Pathways: Tube Formation