ALOX5 antibody (C-Term)

Catalog No. ABIN374732

The Goat Polyclonal anti-ALOX5 antibody is suitable to detect ALOX5 in samples from Human, Mouse, Rat and Dog. It has been validated for WB.

Quick Overview for ALOX5 antibody (C-Term) (ABIN374732)

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This ALOX5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ALOX5 Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts to Arachidonate 5-lipoxygenase.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Canine.
Species reactivity (tested):Human, Rat.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-ERNKKKQLPYYYLSPD, from the C Terminus of the protein sequence Genename: ALOX5

Application Details

Application Notes: Peptide ELISA: 1/32000. Western Blot: 0.3 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ALOX5

Target: ALOX5 (Arachidonate 5-Lipoxygenase (ALOX5))

Alternative Name: ALOX5 / LOG5

Background: The enzyme 5 Lipoxygenase (5 LO) plays a key role in regulating the production of leukotrienes (LTs). Overproduction of LTs contributes to several diseases, most notably chronic inflammatory diseases, including asthma, fibrosis and atherosclerosis. Recent work has demonstrated that the activity of 5 LO is regulated by PKA phosphorylation of serine 523 in 5 LO. Under normal conditions, this phosphorylation may be important in limiting inflammation. Abnormal signaling through cAMP and PKA, then, could contribute to a variety of diseases, including those characterized by chronic inflammation.Synonyms: 5-LO, 5-lipoxygenase, Arachidonate 5-lipoxygenase

Gene ID: 240, 9606

UniProt: P09917