FH antibody (Internal Region)

Catalog No. ABIN374767

This Goat Polyclonal antibody specifically detects FH in WB and EIA. It exhibits reactivity toward Human, Mouse, Rat and Dog.

Quick Overview for FH antibody (Internal Region) (ABIN374767)

Target: FH (Fumarate Hydratase (FH))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This FH antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-FH Antibody

Binding Specificity: Internal Region

Sequence: C-HPNDHVNKSQ SSND

Specificity: This antibody recognizes Fumarase/FH

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat and Canine (Dog).
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_000134.2 Genename: FH

Application Details

Application Notes: Peptide ELISA: 1/64000. Western Blot: 0.01-0.03 μg/mL. Approx 48 kDa band observed in Human Kidney, Liver andLung lysates. The observed molecular weight corresponds to earlier findings withantibodies from different sources. In transfected HEK293 transiently expressing Fumarasea band of approx.58 kDa is observed. This band is not observed in the non-transfectedHEK293.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide and 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for FH

Target: FH (Fumarate Hydratase (FH))

Alternative Name: Fumarase

Background: FH (Fumarate hydratse) catalyzes the conversion of fumarate to malate. It is a key enzyme in the tricarboxylic acid (TCA) cycle. It exists in a cytosolic form and an N terminal extended form. The N terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm. FH also acts as a tumor suppressor.Synonyms: HLRCC, LRCC, MCL, MCUL1, mitochondrial Fumarate hydratase

Molecular Weight: 54.6 kDa (NP_000134.2).

Gene ID: 2271, 9606

UniProt: P07954