PDE11A antibody (Internal Region)

Catalog No. ABIN374774

The Goat Polyclonal anti-PDE11A antibody is suitable to detect PDE11A in samples from Human, Mouse, Rat and Dog. It has been validated for WB.

Quick Overview for PDE11A antibody (Internal Region) (ABIN374774)

Target: PDE11A (phosphodiesterase 11A (PDE11A))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This PDE11A antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PDE11A Antibody

Binding Specificity: Internal Region

Specificity: This antibody reacts to PDE11A.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-NSFKESMEKSSYSD, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/2000. Western Blot: 0.5 - 1.5 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PDE11A

Target: PDE11A (phosphodiesterase 11A (PDE11A))

Alternative Name: PDE11A

Background: One of the many phosphodiesterases that compartmentalize and hydrolyze cAMP and cGMP into AMP and GMP is Phosphodiesterase type 11. The human PDE11A contains an open reading frame encoding a 490 amino acid protein (55-56 kDa). The PDE11A has an N terminus GAF domain homologous to other signaling molecule as found in PDE2, PDE5, PDE6, PDE10, which potentially represents an allosteric cGMP and other small signaling molecules. PDE11A hydrolyzes both cGMP and cAMP, it is sensitive to non selective PDE inhibitor IBMX as well as zaprinast and dipyrimadole inhibitors that are generally selective for cGMP specific enzymes. PDE11A expression is diverse and is found in high levels in skeletal muscle, prostate, kidney, liver, pituitary and testis. There are several isoforms known: Isoform 1 is present in prostate, pituitary, heart and liverbut not in testis or in penis. Isoform 2 is believed to be expressed in testis and Isoform 4 in the adrenal cortex.Synonyms: 5'-cyclic-AMP and -GMP phosphodiesterase 11A, Dual 3', PDE11A1, PDE11A2, PDE11A3, PDE11A4, Phosphodiesterase 11A, cAMP and cGMP phosphodiesterase 11A

Gene ID: 50940

NCBI Accession: NP_001070664

UniProt: Q9HCR9

Pathways: cAMP Metabolic Process