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Perilipin 1 (PLIN1) (C-Term) antibody

Details for Product No. ABIN374794, Supplier: Login to see
Antigen
  • PLIN
  • peri
  • plin
  • perilipin
  • LOC692833
  • FPLD4
  • PERI
  • 6030432J05Rik
  • Peri
  • Plin
  • PERIA
Epitope
C-Term
40
13
11
8
7
3
3
2
2
1
1
1
1
1
1
Reactivity
Dog (Canine), Human, Rat (Rattus)
85
43
41
9
9
7
2
2
1
1
1
Host
Goat
47
39
7
6
3
Clonality
Polyclonal
Conjugate
Un-conjugated
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
88
40
19
13
11
10
5
2
2
Supplier
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Immunogen Syntehtic peptide from the C-terminus of Human PLIN (NP_002657.2) Genename: PLIN
Sequence C-EPILGRAQYS QLRKK
Specificity This antibody detects Perilipin-1 at C-term.
Cross-Reactivity (Details) Species reactivity (expected):Rat, Dog.
Species reactivity (tested):Human.
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Alternative Name Perilipin-1 (PLIN1 Antibody Abstract)
Background Synonyms: Lipid Droplet-Associated Protein, PERI, PLIN, PLIN-1, PLIN1, Perilipin
Gene ID 5346, 9606
UniProt O60240
Research Area Metabolism
Pathways
Application Notes Peptide ELISA: antibody detection limit dilution 1: 32000. Western blot: 0.01-0.03 μg/mL. Approx 55-60 kDa band observed in Human Adipose lysates(calculated MW of 56.0 kDa according to NP_002657.2). Immunofluorescence: 1 μg/mL. Small lipid droplets preferentially stained in the periphery ofdifferentiated 3T3-L1 adipocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.5 mg/mL
Buffer Tris saline, pH 7.3 containing 0.02 % Sodium Azide and 0.5 % BSA
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C.
Supplier Images
 image for anti-Perilipin 1 (PLIN1) (C-Term) antibody (ABIN374794) anti-Perilipin 1 (PLIN1) (C-Term) antibody
 image for anti-Perilipin 1 (PLIN1) (C-Term) antibody (ABIN374794) anti-Perilipin 1 (PLIN1) (C-Term) antibody (Image 2)
Background publications Wolins, Brasaemle, Bickel: "A proposed model of fat packaging by exchangeable lipid droplet proteins." in: FEBS letters, Vol. 580, Issue 23, pp. 5484-91, 2006 (PubMed).

Garcia-Carbonero, Supko, Maki et al.: "Ecteinascidin-743 (ET-743) for chemotherapy-naive patients with advanced soft tissue sarcomas: multicenter phase II and pharmacokinetic study." in: Journal of clinical oncology : official journal of the American Society of Clinical Oncology, Vol. 23, Issue 24, pp. 5484-92, 2005 (PubMed).