Kallikrein 5 antibody (Internal Region)

Catalog No. ABIN374828

The Goat Polyclonal anti-Kallikrein 5 antibody is suitable to detect Kallikrein 5 in samples from Human, Rat and Dog. It has been validated for WB.

Quick Overview for Kallikrein 5 antibody (Internal Region) (ABIN374828)

Target: Kallikrein 5 (KLK5)

Reactivity: Human, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This Kallikrein 5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-Kallikrein 5 Antibody

Binding Specificity: Internal Region

Specificity: This antibody recognizes KLK5.

Cross-Reactivity (Details): Species reactivity (expected):Rat and Canine.
Species reactivity (tested):Human.

Purification: Affinity Chromatography.

Immunogen: Peptide with sequence KAGRDSCQGD, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/4000. Western Blot: 0.1-0.3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Kallikrein 5

Target: Kallikrein 5 (KLK5)

Alternative Name: KLK5 / Kallikrein-5

Background: Kallikrein 5 was originally described from the skin as an enzyme involved in turnover of the stratum corneum, and later cloned from human EST libraries as a Kallikrein like enzyme. The skin is known to contain trypsin like and chymotrypsin like proteinases of 32 kDa and 25 kDa respectively, and these were determined to be hK5 and hK7. Kallikrein 5 is found is greatest abundance in the skin, and also in the brain, with lesser signal in the kidney, lung, mammary gland, and testis. Human breast milk is reported to contain high levels of Kallikrein 5. Less stringent PCR detects hK5 in many tissues, and analysis of cell lines by Western blot shows that many cell lines make hK5.Synonyms: KLK-5, KLK-L2, Kallikrein-Like Protein 2, SCTE, Stratum corneum tryptic enzyme

Gene ID: 25818

NCBI Accession: NP_001070959

UniProt: Q9Y337

Pathways: Complement System, Regulation of G-Protein Coupled Receptor Protein Signaling