APPL1 antibody (Internal Region)

Catalog No. ABIN374837

The Goat Polyclonal anti-APPL1 antibody has been validated for WB. It is suitable to detect APPL1 in samples from Human, Mouse, Rat, Cow and Dog.

Quick Overview for APPL1 antibody (Internal Region) (ABIN374837)

Target: APPL1 (Adaptor Protein, phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1))

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This APPL1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-APPL1 Antibody

Binding Specificity: Internal Region

Sequence: C-NRYSRLSKKR ENDK

Specificity: This antibody recognizes APPL1.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine, Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_036228.1.

Application Details

Application Notes: Peptide ELISA: 1/64000. Western Blot: 0.3-1 μg/mL. Approx 80 kDa band observed in Rat Aortic Smooth SkeletalMuscle Cells (lane 1), intensity increased after infection with Human APPL1 adenovirus for48h (lane 2). Calculated MW of 79.0 kDa according to NP_036228.1. The blot wasco-labeled with Tubulin-specific antibodies as a loading control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for APPL1

Target: APPL1 (Adaptor Protein, phosphotyrosine Interaction, PH Domain and Leucine Zipper Containing 1 (APPL1))

Alternative Name: APPL1 / DIP13A

Background: APPL is involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The APPL binds to RAB5A/Rab5 through N-terminal domain. This interaction is essential for its recruitment to endosomal membranes as well as its role in cell proliferation.Synonyms: Adapter protein containing PH domain, DCC-interacting protein 13-alpha, Dip13-alpha, KIAA1428, PTB domain and leucine zipper motif 1

Gene ID: 26060, 9606

UniProt: Q9UKG1