ABHD5 antibody (Internal Region)

Catalog No. ABIN374842

This Goat Polyclonal antibody specifically detects ABHD5 in WB. It exhibits reactivity toward Human, Mouse, Rat and Dog.

Quick Overview for ABHD5 antibody (Internal Region) (ABIN374842)

Target: ABHD5 (Abhydrolase Domain Containing 5 (ABHD5))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This ABHD5 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ABHD5 Antibody

Binding Specificity: Internal Region

Specificity: This antibody recognizes CGI58 / ABHD5. AP16647PU-N is not expected to cross-react with ABHD4.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat and Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography.

Immunogen: Peptide with sequence C-FPERPDLADQDR, from the internal region of the protein sequence Genename: ABHD5

Application Details

Application Notes: Peptide ELISA: ABHD5 Antibody detection limit dilution: 1/16000. Western Blot: 0.2-0.6 μg/mL. Approx 40 kDa band observed in lysates of cell lines NIH3T3and A431 calculated MW of 39.0 kDa according to NP_057090.2). Additional faint bands of20 kDa and 10 kDa were observed. These bands were successfully blocked by incubationwith the immunizing peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ABHD5

Target: ABHD5 (Abhydrolase Domain Containing 5 (ABHD5))

Alternative Name: ABHD5

Background: ABHD5 belongs to a large family of proteins defined by an alpha/beta hydrolase fold, and contains three sequence motifs that correspond to a catalytic triad found in the esterase/lipase/thioesterase subfamily. It differs from other members of this subfamily in that its putative catalytic triad contains an asparagine instead of the serine residue. Mutations in this gene have been associated with Chanarin-Dorfman syndrome, a triglyceride storage disease with impaired long-chain fatty acid oxidation. ABHD5 is widely expressed in various tissues, including skin, lymphocytes, liver, skeletal muscle and brain.Synonyms: Abhydrolase domain-containing protein 5, CGI-58, NCIE2

Gene ID: 51099, 9606

UniProt: Q8WTS1

Pathways: Lipid Metabolism