ADH5 antibody (Internal Region)

Catalog No. ABIN374869

This Goat Polyclonal antibody specifically detects ADH5 in WB, IHC (p) and EIA. It exhibits reactivity toward Human, Mouse, Rat, Pig, Cow and Dog.

Quick Overview for ADH5 antibody (Internal Region) (ABIN374869)

Target: ADH5 (Alcohol Dehydrogenase 5 (Class III), chi Polypeptide (ADH5))

Reactivity: Human, Mouse, Rat, Pig, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This ADH5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-ADH5 Antibody

Binding Specificity: Internal Region

Sequence: C-KKIKVDEFVT HN

Specificity: This antibody detects Alcohol Dehydrogenase 5 (ADH5).

Cross-Reactivity (Details): Species reactivity (expected):Canine (Dog), Pig, Bovine.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_000662.3 Genename: ADH5

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 1000. Western blot: 1-3 μg/mL. Customer's experiments gave a band at approx 38 kDa in MouseLymphocyte lysates (calculated MW of 39.8 kDa according to Human NP_000662.3 and39.8 kDa according to Mouse NP_031436.2).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ADH5

Target: ADH5 (Alcohol Dehydrogenase 5 (Class III), chi Polypeptide (ADH5))

Alternative Name: Alcohol Dehydrogenase 5 (ADH5)

Background: The ADH5 gene encodes glutathione dependent formaldehyde dehydrogenase or class III alcohol dehydrogenase chi subunit, which is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, retinol, ethanol and other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class III alcohol dehydrogenase is a homodimer composed of 2 chi subunits. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long chain primary alcohols and for oxidation of S hydroxymethyl glutathione, a spontaneous adduct between formaldehyde and glutathione. The enzyme is an important component of cellular metabolism for the elimination of formaldehyde, which is a potent irritant and sensitizing agent that causes rhinitis, lacrymation, contact dermatitis and pharyngitis.Synonyms: Alcohol dehydrogenase class 3 chi chain, Glutathione-dependent formaldehyde dehydrogenase, S-(hydroxymethyl)glutathione dehydrogenase

Molecular Weight: 39.7 kDa according to NP_000662.3.

Gene ID: 128, 9606

UniProt: P11766