IGFBP3 antibody (Internal Region)

Catalog No. ABIN374871

This anti-IGFBP3 antibody is a Goat Polyclonal antibody detecting IGFBP3 in WB. Suitable for Human, Mouse, Rat and Dog.

Quick Overview for IGFBP3 antibody (Internal Region) (ABIN374871)

Target: IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This IGFBP3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-IGFBP3 Antibody

Binding Specificity: Internal Region

Sequence: C-RYKVDYESQS TDTQN

Specificity: This antibody detects IGFBP3. It is expected to recognise both reported isoforms (NP_001013416.1 and NP_000589.2).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, DogSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_001013416.1, NP_000589.2.

Application Details

Application Notes: Peptide ELISA: antibody detection limit dilution 1: 64000. Western blot: 0.02-0.06 μg/mL. Approx 28 kDa band observed in Human Breast cancerlysates (calculated MW of 31.8 kDa according to NP_001013416.1 and 32.8 kDa according toNP_000589.2). An additional band of 58 kDa was consistently observed, however this bandwas not blocked by the immunizing peptide and it is therefore a non-specific signal. Wecall for caution when used for other assays than Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for IGFBP3

Target: IGFBP3 (Insulin-Like Growth Factor Binding Protein 3 (IGFBP3))

Alternative Name: IGFBP3

Background: Insulin like growth factor binding protein 3 (IGFBP3) is a member of the superfamily of insulin like growth factor (IGF) binding proteins which include six high affinity IGF binding proteins (IGFBP) and at least four low affinity binding proteins referred to as IGFBP related proteins (IGFBPrP). The IGFBP members are cysteine rich proteins with conserved cysteine residues clustered in the amino terminal and the carboxy terminal regions of the molecule. The cDNA sequence encoding the mature human IGFBP3 is fused to the signal peptide of CD33. Human IGFBP3 is the major IGF binding protein in plasma where it exists in a ternary complex with IGFI or IGFII and an acid labile subunit. IGFBPs hold a central position in IGF ligand-receptor interactions through influences on both the bioavailability and distribution of IGFs in the extracellular environment. Insulin like growth factor binding protein 3 (IGFBP3) can modulate the mitogenic and metabolic effects of the insulin like growth factors (IGFs). Insulin like growth factor binding protein 3 (IGFBP3) is expressed in multiple tissues. The highest expression level is found in the non paranchymal cells of the liver. The expression levels are higher during extrauterine life and peak during puberty. Insulin like growth factors (IGFs) and IGF binding proteins (IGFBPs) play important roles in cell growth and differentiation. IGFBP3 is one of the factors in serum that is responsible for high serum induced apoptosis in PC3 cells, a prostate cancer cell line. IGFBP3 is important in controlling glucose homeostasis with increased urinary levels in type I diabetes with persistent microalbuminuria.Synonyms: IBP-3, IBP3, IGF-BP3, IGF-binding protein 3, IGFBP-3, Insulin-like growth factor-binding protein 3

Gene ID: 3486, 9606

UniProt: P17936

Pathways: Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding