F13A1 antibody (Internal Region)

Catalog No. ABIN374910

The Goat Polyclonal anti-F13A1 antibody (ABIN374910) specifically detects F13A1 in WB. The antibody is reactive with Human and Mouse samples.

Quick Overview for F13A1 antibody (Internal Region) (ABIN374910)

Target: F13A1 (Coagulation Factor XIII, A1 Polypeptide (F13A1))

Reactivity: Human, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This F13A1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-F13A1 Antibody

Binding Specificity: AA 703-717, Internal Region

Specificity: This antibody reacts to Factor XIIIa.

Cross-Reactivity (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-HRKLIASMSSDSLRH, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/32000. Western Blot: 0.05 - 0.2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for F13A1

Target: F13A1 (Coagulation Factor XIII, A1 Polypeptide (F13A1))

Alternative Name: Coagulation Factor XIIIa (F13A1)

Background: Factor XIII is a beta globulin found in plasma and is composed of two subunits. Coagulation factor XIII is the last zymogen to become activated in the blood coagulation cascade. Factor XIII A is the catalytic subunit and is a dimmer of molecular weight 160 kDa. The B subunits do not have enzymatic activity and may serve as a plasma carrier molecules. Platelet factor XIII is comprised only of 2 A subunits, which are identical to those of plasma origin. Upon activation by the cleavage of the activation peptide by thrombin and in the presence of calcium ion, the plasma factor XIII dissociates its B subunits and yields the same active enzyme, factor XIII A, as platelet factor XIII. This enzyme acts as a transglutaminase to catalyze the formation of gamma-glutamyl-epsilon-lysine crosslinking between fibrin molecules, thus stabilizing the fibrin clot. It also crosslinks alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin. Factor XIII deficiency is classified into two categories: type I deficiency, characterized by the lack of both the A and B subunits, and type II deficiency, characterized by the lack of the A subunit alone. These defects can result in a lifelong bleeding tendency, defective wound healing, and habitual abortion. Factor XIII A is a dermal dendrocyte marker and shows variable reaction with these types of tumors. It can be used for histiocytic phenotyping and has been reported to mark capillary hemangiomas and tumors of the central nervous system.Synonyms: Coagulation factor XIII A chain, F13A, Protein-glutamine gamma-glutamyltransferase A chain, Transglutaminase A chain

Gene ID: 2162

NCBI Accession: NP_000120

UniProt: P00488