GOT2 antibody (Internal Region)

Catalog No. ABIN374942

The Goat Polyclonal anti-GOT2 antibody is suitable to detect GOT2 in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for GOT2 antibody (Internal Region) (ABIN374942)

Target: GOT2 (Glutamic-Oxaloacetic Transaminase 2, Mitochondrial (Aspartate Aminotransferase 2) (GOT2))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This GOT2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-GOT2 Antibody

Binding Specificity: Internal Region

Specificity: This antibody reacts to GOT2.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence CKDADEAKRVES, from the internal region of the protein sequence

Application Details

Application Notes: Peptide ELISA: 1/32000. Western Blot: 0.03 - 0.1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GOT2

Target: GOT2 (Glutamic-Oxaloacetic Transaminase 2, Mitochondrial (Aspartate Aminotransferase 2) (GOT2))

Alternative Name: GOT2

Background: GOT2 [Glutamate oxaloacetate transaminase] is a ubiquitous pyridoxal phosphate dependent enzyme which exists in both mitochondrial and cytosolic forms. The enzyme plays an important role in amino acid metabolism and in the urea and tricarboxylic acid cycles. The 2 isoenzymes are homodimeric. In liver about 80 % of the enzyme activity is mitochondrial in origin, whereas in serum the enzyme activity is largely cytosolic. Although the mitochondrial and soluble forms of GOT are coded by different chromosomes, the 2 show close homology in amino acid sequence and were presumably derived from a common ancestral gene. Serum GOT [with SGPT] levels are usually elevated in states of hepatocellular injury (injury to the liver cells), the highest levels are associated with hepatitis of a viral origin. High levels are also found after myocardial infarction, when SGPT levels are lower.Synonyms: Aspartate aminotransferase, FABP-1, FABPpm, Fatty acid-binding protein, Transaminase A, mAspAT, mitochondrial

Gene ID: 2806, 9606

UniProt: P00505

Pathways: Monocarboxylic Acid Catabolic Process