LASS3 antibody (C-Term)

Catalog No. ABIN375017

The Goat Polyclonal anti-LASS3 antibody is suitable to detect LASS3 in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for LASS3 antibody (C-Term) (ABIN375017)

Target: LASS3 (CERS3) (Ceramide Synthase 3 (CERS3))

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This LASS3 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-LASS3 Antibody

Binding Specificity: C-Term

Sequence: C-RAERHLIPNG QHGH

Specificity: Recognizes LASS3 / CerS3.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Human.
Species reactivity (tested):Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the C-Terminus of the protein sequence according to NP_849164.2 Genename: LASS3

Application Details

Application Notes: Peptide ELISA: 1/32000. Western Blot: 2 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for LASS3

Target: LASS3 (CERS3) (Ceramide Synthase 3 (CERS3))

Alternative Name: LASS3

Background: LASS3 (Longevity Assurance homolog 3) is a member of a highly conserved family of proteins from yeast to humans which are involved in the synthesis of ceramide. Overproduction of LASS3 appears to results in increases in several ceramide species, with some preference toward those having middle to long chain fatty acid acyl-CoAs. Also appears to have (dihydro)ceramide synthesis activity. LASS3 expression appears to be limited almost solely to the testis.Synonyms: LAG1 longevity assurance homolog 3

Gene ID: 204219, 9606

UniProt: Q8IU89