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Antibodies
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anti-zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) antibody (FITC)
zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70) antibody (FITC)
| Antigen |
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| Synonyms |
SRK, STD, TZK, ZAP-70, FLJ17670, FLJ17679, Srk, mur, 70kDa, mrtle, AI327364, MGC108902, zap70, MGC80670, MGC88962, ZAP70 |
| Clonality |
Monoclonal (SBZAP) |
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Host
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Reactivity
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Conjugate
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Biotin (6), FITC (5), PE (3), HRP (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Alkaline Phosphatase (AP) (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1), RPE (1)
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Application
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Western Blotting (WB) (79), Flow Cytometry (FACS) (36), Immunohistochemistry (IHC) (33), Immunofluorescence (IF) (32), ELISA (30), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (27), Enzyme Immunoassay (EIA) (12), Immunoprecipitation (IP) (11), Immunocytochemistry (ICC) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1)
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| Catalog no. |
ABIN375466 |
| Quantity |
0.025 mg (Variants) |
| Price |
115.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Alternative name
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ZAP-70
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Isotype
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IgG1
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Clone
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SBZAP
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Specificity
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Human TCR Zeta, Zap-70 (Mr 70 kDa) ZAP 70, also known as TCR associated protein, is a protein tyrosine kinase (PTK) with the primary role of catalyzing the phosphorylation of tyrosine residues in cellular proteins, and thus, contributes significantly to the initiation of cell signaling. ZAP 70 is expressed on T cells and NK cells. The monoclonal antibodies SB70 and SBZAP were generated against a KLH-peptide sequence corresponding to the human ZAP 70 amino acid residues 280-309.
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Application Notes
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To insure lot-to-lot consistency, each batch of product is tested by ELISA and/or PCFIA assay to conform to the characteristics of a standard reference reagent. Purified antibody conjugate: <= 1 µg/10 6 cells, FITC conjugate: <= 1 µg/10 6 cells, R-PE conjugate: <= 0.2 µg/10 6 cells, AP conjugate: 1:500-1:1,000, HRP conjugate: 1:1,000-1:2,000.
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Buffer
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The purified antibody is supplied as 0.025 mg purified immunoglobulin in 0.25 mL or 0.1 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added . Store at 2-8°C. The fluoresc
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Storage
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Store at 2-8°C
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Research Area
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Signaling, Tags/Labels, Immunology, Cancer
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Restrictions
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For Research Use only
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Alternatives for antigen "zeta-Chain (TCR) Associated Protein Kinase 70kDa (ZAP70)", type "Antibodies"
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Hosts
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Rabbit (100), Mouse (41)
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Reactivities
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Human (136), Mouse (Murine) (66), Rat (Rattus) (60), Cow (Bovine) (18), Pig (Porcine) (18), Dog (Canine) (17), Horse (Equine) (16), Rabbit (16), Chicken (15), Guinea Pig (15)
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Applications
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Western Blotting (WB) (79), Flow Cytometry (FACS) (36), Immunohistochemistry (IHC) (33), Immunofluorescence (IF) (32), ELISA (30), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (27), Enzyme Immunoassay (EIA) (12), Immunoprecipitation (IP) (11), Immunocytochemistry (ICC) (4), Immunohistochemistry (Frozen Sections) (IHC (fro)) (3), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (2), ELISA (Detection) (1), Immunoelectron Microscopy (IEM) (1)
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Conjugates
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Biotin (6), FITC (5), PE (3), HRP (2), Alexa Flour 488 (1), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Alkaline Phosphatase (AP) (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), Gold (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1), RPE (1)
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Epitopes
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pTyr319 (10), pTyr493 (8), Ab-319 (5), Ab-493 (5), C-Term (5), Center (3), Ab-292 (1), Ab-315 (1), pTyr292 (1), pTyr315 (1), pTyr492 (1)
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Alternatives 
