Goat anti-Mouse (Murine) IgG Antibody (HRP)

Details for Product No. ABIN376241
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Antigen
Reactivity
Mouse (Murine)
(1891), (1864), (1539), (1210), (969), (496), (435), (343), (298), (266), (266), (260), (225), (222), (122), (93), (85), (81), (32), (28), (21), (14), (8), (8), (7), (5), (5), (4), (3), (3), (2), (1), (1), (1)
Host
Goat
(4776), (3558), (1178), (463), (396), (254), (67), (43), (25), (8), (5), (1)
Clonality
Polyclonal
Conjugate
HRP
(1514), (1376), (1246), (1003), (674), (553), (413), (201), (199), (116), (115), (113), (111), (98), (98), (33), (32), (21), (20), (15), (11), (10), (10), (10), (10), (10), (10), (10), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (8), (7), (6), (6), (5), (5), (5), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Pubmed 4 references available
Quantity 1 mL
Options
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Catalog No. ABIN376241
154.00 $
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Isotype IgG
Specificity Reacts with mouse IgG 1, IgG 2a, IgG 2b and IgG 3 as demonstrated by ELISA and flow cytometry.
Characteristics Source: Pooled antisera from goats hyperimmunized with mouse IgG paraproteins.
To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry for conformance with characteristics of a standard reference reagent.
Purification Affinity chromatography on mouse IgG 3 covalently linked to agarose.
Research Area Immunology, Secondary Antibodies
Application Notes Working Dilution:
1:4,000-1:8,000
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Product cited in: Kim, Welsch, Yi et al.: "Regulation of the production of infectious genotype 1a hepatitis C virus by NS5A domain III." in: Journal of virology, Vol. 85, Issue 13, pp. 6645-56, 2011 (PubMed).

Gao, Yallampalli, Yallampalli: "Gestational Protein Restriction Increases Angiotensin II Production in Rat Lung." in: Biology of reproduction, Vol. 88, Issue 3, pp. 64, 2013 (PubMed).

Kamphuis, Shafique, Meijerhof et al.: "Efficacy and safety of an intranasal virosomal respiratory syncytial virus vaccine adjuvanted with monophosphoryl lipid A in mice and cotton rats." in: Vaccine, 2013 (PubMed). Further details: Plates were incubated with a 1:5000 dilution of horseradish-peroxidase-coupled goat anti-mouse IgG, or IgA which bind to both mouse and cotton rat IgG and IgA, respectively

General Chen, Lin, Wang: "Amyloid P component as a plasma marker for Parkinson's disease identified by a proteomic approach." in: Clinical biochemistry, 2011 (PubMed).

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