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Goat anti-Mouse (Murine) IgG Antibody (HRP)

Details for Product No. ABIN376241, Supplier: Login to see
Antigen
Reactivity
Mouse (Murine)
2413
2330
2036
1524
1260
609
562
383
328
296
295
291
290
224
162
112
105
76
32
24
21
13
12
8
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
Host
Goat
6021
4118
1527
608
545
262
85
49
43
8
8
4
4
3
1
1
Clonality
Polyclonal
Conjugate
HRP
1869
1685
1511
1095
605
523
517
261
198
181
172
161
156
155
127
79
79
69
43
41
37
32
32
31
23
20
19
19
17
15
14
14
14
14
13
10
10
10
10
10
10
9
9
9
9
8
8
8
8
8
8
8
8
8
8
7
7
6
6
6
6
6
6
5
5
5
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
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Isotype IgG
Specificity Reacts with mouse IgG 1, IgG 2a, IgG 2b and IgG 3 as demonstrated by ELISA and flow cytometry.
Characteristics Source: Pooled antisera from goats hyperimmunized with mouse IgG paraproteins.
To insure lot-to-lot consistency, each batch of product is tested by ELISA, PCFIA and/or flow cytometry for conformance with characteristics of a standard reference reagent.
Purification Affinity chromatography on mouse IgG 3 covalently linked to agarose.
Research Area Immunology, Secondary Antibodies
Application Notes Working Dilution:
1:4,000-1:8,000
Representative data are included in this product insert.

Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Product cited in: Kamphuis, Shafique, Meijerhof et al.: "Efficacy and safety of an intranasal virosomal respiratory syncytial virus vaccine adjuvanted with monophosphoryl lipid A in mice and cotton rats." in: Vaccine, 2013 (PubMed). Further details: Plates were incubated with a 1:5000 dilution of horseradish-peroxidase-coupled goat anti-mouse IgG, or IgA which bind to both mouse and cotton rat IgG and IgA, respectively

Gao, Yallampalli, Yallampalli: "Gestational protein restriction increases angiotensin II production in rat lung." in: Biology of reproduction, Vol. 88, Issue 3, pp. 64, 2013 (PubMed).

Kim, Welsch, Yi et al.: "Regulation of the production of infectious genotype 1a hepatitis C virus by NS5A domain III." in: Journal of virology, Vol. 85, Issue 13, pp. 6645-56, 2011 (PubMed).

Chen, Lin, Wang: "Amyloid P component as a plasma marker for Parkinson's disease identified by a proteomic approach." in: Clinical biochemistry, 2011 (PubMed).