IL2 Receptor beta antibody

Catalog No. ABIN376384

This Rat Monoclonal antibody specifically detects IL2 Receptor beta in FACS and IP. It exhibits reactivity toward Mouse.

Quick Overview for IL2 Receptor beta antibody (ABIN376384)

Target: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This IL2 Receptor beta antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: 5H4

Product Details anti-IL2 Receptor beta Antibody

Purpose: Rat Anti-Mouse CD122-LE/AF

Specificity: Mouse CD122, 90-100 kDa

Characteristics: Low Endotoxin/Azide Free Anti-Mouse CD122 antibody for use in flow cytometry and immunoprecipitation assays.

Endotoxin Level: Low Endotoxin

Immunogen: Rat myeloma YB2/0 transfected with truncated IL-2Rbeta cDNA (YB2/0-m-beta-t-28)

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate buffered saline, pH 7.4

Preservative: Azide free

Handling Advice: Each reagent is stable for the period shown on the bottle label if stored as directed.

Storage: 4 °C

Storage Comment: 2-8°C

Target Details for IL2 Receptor beta

Target: IL2 Receptor beta (IL2RB) (Interleukin 2 Receptor, beta (IL2RB))

Alternative Name: CD122

Background: The IL-2 receptor is a complex of three distinct polypeptide chains: (i) the α chain which binds IL-2 with low affinity, (ii) the β chain that binds IL-2 with high affinity, and (ii) the common γ chain ( γ C ) that does not bind IL-2. The high affinity receptor complex is an α / β / γ heterotrimer with a K d of 1.3 x 10 -11 M. In mouse spleen, CD122 is expresed on approx. 30 % of CD8 + cells and all NK cells, but < 1 % of B cells and CD4 + T lymphocytes. In the thymus, its expression is confined to CD4 - CD8 + single positive and CD4 - CD8 - double negative cells. Cytoplasmic regions of the IL-2R β chain are involved in IL-2-mediated cellular signaling and, via the interaction of IL-2 and its receptor complex, may be involved in the generation and differentiation of T lymphocytes.

Gene ID: 16185

UniProt: P16297

Pathways: JAK-STAT Signaling, Growth Factor Binding