Donkey anti-Goat IgG (H&L) Antibody

Antigen: IgG (H&L)

Clonality: Polyclonal

Host: Donkey

Reactivity: Goat

Conjugate: Un-conjugated

Application: ELISA, Immunofluorescence (IF)

Catalog no.: ABIN376977

Additional Information

Alternative name: IgG(H+L)

Immunogen: Source: Pooled antisera from donkey hyperimmunized with goat IgG. Cross Absorption:Source: Pooled antisera from donkey hyperimmunized with goat IgG.

Isotype: IgG

Specificity: Reacts with the heavy and light chains of Goat IgG as demonstrated by ELISA. May also react with the light chains of other goat immunoglobulins.

Application Details

Application Notes: To insure lot-to-lot consistency, each batch of product is tested by ELISA and/or PCFIA for conformance to characteristics of a standard reference reagent. FITC conjugate: <= 1 µg/10 6 cells, BIOT conjugate: <= 1 µg/10 6 cells, R-PE conjugate: <= 0.1 µg/10 6 cells, AP conjugate: 1:2,000-1:4,000, HRP conjugate: 1:4,000-1:8,000, BIOT conjugate: 1:5,000-1:20,000.

Purification: Cross Absorption: Minimal cross reaction to rat and mouse serum proteins Purification:Affinity chromatography on Goat IgG covalently linked to agarose.

Buffer: The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8°C. The fluorescein (FITC) conjugate is supplied as 1.0 mg

Storage: Store at 2-8°C

Restrictions: For Research Use only