DYKDDDDK antibody (HRP)
| Antigen |
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| Clonality |
Monoclonal (5A8E5) |
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Host
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Conjugate
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Application
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 Alternatives ELISA, Western Blotting (WB), Immunofluorescence (IF)
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| Catalog no. |
ABIN387715 |
| Quantity |
100ug (0.5 mg/ml, lyophilized with PBS, pH...) |
| Price |
149.00 $ Plus shipping costs $35.00
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| Shipping to |
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| Availability |
Ships within 5 to 7 Business Days |
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Immunogen
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A synthetic peptide (DYKDDDDK) coupled to KLH
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Format
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Purified
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Isotype
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IgG2b, kappa chain
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Clone
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5A8E5
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Description
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Antibodies-Online Anti-DYKDDDDK [HRP] Monoclonal Antibody (Mouse) is HRP conjugated THETM Anti-DYKDDDDK Monoclonal Antibody (Mouse) (ABIN387700) purified from mice ascites by protein A affinity column. The antibody is suitable for detecting the expression level of DYKDDDDK in fusion proteins.
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Specificity
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Anti-DYKDDDDK [HRP] Monoclonal Antibody (Mouse) recognizes C-terminal, N-terminal, and internal tagged fusion proteins.
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Application Notes
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 0.05-0.2 ug/ml Western blot: 0.1-1.0 ug/ml Other applications: user-optimized
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Concentration
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0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.01% thimerosal
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Purification
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Protein A affinity column
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Storage
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Mouse Anti-DYKDDDDK-tag Monoclonal Antibody (HRP) should be stored lyophilized until use. It is stable in lyophilized form for at least two years if stored at -20°C. The reconstituted antibody can be stored for up to three months at 2-8°C or for up to two years at -20°C or below. Avoid repeated freeze and thaw cycles.
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Research Area
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Tags/Labels
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Restrictions
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For Research Use only
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Primary antibody:A. 1 µg/ml Anti-alpha-tubulin Monoclonal Antibody (Mouse) (Antibodies-Online, Cat. No. ABIN387714, Lot No. A206907)B. 1 µg/ml mouse anti-alpha-tubulin mAb (competitor A)Secondary antibody:IRDye 800 Conjugated affinity Purified Goat Anti-Mouse IgG (ROCKLAND, 1:10,000, 610-132-121)Loading:1.20 µg HeLa cell lysate2. 4 µg HeLa cell lysate3. 0.8 µg HeLa cell lysate
Primary antibody:1 µg/ml Anti-alpha-tubulin Monoclonal Antibody (Mouse) (Antibodies-Online, Cat.No. ABIN387714, Lot.No. A206907Secondary antibody:IRDye 800 Conjugated affinity Purified Goat Anti-Mouse IgG (ROCKLAND, 1:10,000, 610-132-121)Loading:1. HeLa cell lysate 2. NIH/3T3 cell lysate3. CHO cell lysate4. COS-7 cell lysate5. Pig muscle cell lysate
Immunofluorescence analysis of 293 cellsPrimay antibody: 1 µg/ml Anti-alpha tubulin Monoclonal Antibody (Mouse) (Antibodies-Online, ABIN387714)Secondary antibody: Fluorescein Conjugated Affinity Purified Anti-Mouse IgG (H&L) (Goat) (Rockland, 1:1,000, 610-102-121)
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Alternatives for antigen "DYKDDDDK", type "Antibodies"
