DYKDDDDK antibody (Biotin)

Antigen: DYKDDDDK

Clonality: Monoclonal (5A8E5)

Host: Mouse

Conjugate: Biotin

Application: ELISA, Western Blotting (WB)

Certificates: ISO 9001:2008

Catalog no.: ABIN387716

Additional Information

Immunogen: A synthetic peptide (DYKDDDDK) coupled to KLH

Format: Purified

Isotype: IgG2b, kappa chain

Clone: 5A8E5

Description: Antibodies-Online Anti-DYKDDDDK [Biotin] Monoclonal Antibody (Mouse) is the biotin conjugated Anti-DYKDDDDK Monoclonal Antibody (Mouse) (ABIN387700) purified from mice ascites by protein A affinity column. The antibody is suitable for detecting the expression level of DYKDDDDK in fusion proteins.

Specificity: Anti-DYKDDDDK [Biotin] Monoclonal Antibody (Mouse) recognizes C-terminal, N-terminal, and internal tagged fusion proteins.

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.ELISA: 0.05-0.2 ug/ml Western blot: 0.1-1.0 ug/ml Other applications: user-optimized

Concentration: 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide

Purification: Protein A affinity column

Storage: Mouse Anti-DYKDDDDK-tag Monoclonal Antibody (Biotin) should be stored lyophilized until use. It is stable in lyophilized form for at least two years if stored at -20°C. The reconstituted antibody can be stored for up to three months at 2- 8°C or for up to two years at -20°C or below. Avoid repeated freeze and thaw cycles.

Research Area: Tags/Labels

Restrictions: For Research Use only

Images

Loading: Multiple Tag (Purified) (Antibodies-Online, Cat.No. M0101, 400 ng, 80 ng, 16 ng)Primary antibody: 1 µg /ml Mouse Anti-DYKDDDDK-tag Monoclonal Antibody (HRP) (Antibodies-Online, Cat. No. ABIN387715, Lot No. A213902)