DYKDDDDK antibody (FITC)

Antigen: DYKDDDDK

Clonality: Monoclonal (5A8E5)

Host: Mouse

Conjugate: FITC

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Certificates: ISO 9001:2008

Catalog no.: ABIN387724

Additional Information

Immunogen: A synthetic peptide DYKDDDDK coupled to KLH

Format: Purified

Isotype: IgG2b, kappa chain

Clone: 5A8E5

Description: Antibodies-Online Anti-DYKDDDDK [FITC] Monoclonal Antibody is FITC conjugated Anti-DYKDDDDK-Tag Monoclonal Antibody (Mouse) (ABIN387715) purified from mice ascites by Protein A affinity column. This product is suitable for detecting the expression level of DYKDDDDK fusion proteins by various immunoassays. Absorption Wavelength: 492 nmEmission Wavelength: 518 nmFluorochrome/Protein Ratio: >3 moles FITC per mole antibody

Specificity: Antibodies-Online Anti-DYKDDDDK [FITC] Monoclonal Antibody (Mouse) recognizes C-terminal, N-terminal, and internal DYKDDDDK tagged fusion proteins.

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. Immunofluorescence (IF): 0.5-2 ug/mlFlow cytometry: 0.5-2 ug for 1 x 106 cellsOther applications: User-optimized

Storage: GenScript Anti-DYKDDDDK [FITC] Monoclonal Antibody (Mouse) should be stored lyophilized until use. It is stable in lyophilized form for at least two years if stored at -20°C. The reconstituted antibody can be stored for up to three months at 2-8°C or for up to two years at -20°C or below. Avoid repeated freeze and thaw cycles.

Research Area: Tags/Labels

Restrictions: For Research Use only

Images

Flow cytometric analysis of transfected HEK-293 cell expressing HA-tagged protein using Anti-HA-tag [FITC] Monoclonal Antibody (Antibodies-Online, ABIN387723, shaded histogram) or with a negative control antibody (open histogram).