Autophagy Related 12 (ATG12) antibody

Details for Product No. ABIN387794
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Antigen
Synonyms APG12, APG12L, FBR93, HAPG12, 4931423H11Rik, A330058M13Rik, Apg12l, Atg12l
Reactivity
Human
(95), (42), (41), (12), (12), (12), (4), (1), (1)
Host
Mouse
(91), (13), (4), (1)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(5), (5), (5), (4), (4), (4), (1), (1), (1), (1), (1), (1), (1), (1)
Application
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
(86), (62), (53), (13), (13), (11), (10), (5), (1), (1)
Pubmed 5 references available
Quantity 400 μL
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Catalog No. ABIN387794
291.50 $
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Immunogen APG12L recombinant protein is used to produce this monoclonal antibody.
Clone 43CT73-3-5-5-4
Isotype IgG2b kappa
Specificity APG12L recombinant protein is used to produce this monoclonal antibody.
Purification This antibody is purified through a protein A column, followed by dialysis against PBS.
Alternative Name ATG12
Background Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).
Synonyms: APG12, APG12L, FBR93, HAPG12, APG12 autophagy 12-like, APG12-like, Apg12 (autophagy, yeast) homolog, autophagy-related protein 12
Molecular Weight 15113 DA
Gene ID 9140
UniProt O94817
Research Area Apoptosis/Necrosis, Autophagy, Cell Structure
Application Notes WB = 1:200-2000, IHC = 1:50-200, IF = 1:10-50
Restrictions For Research Use only
Format Liquid
Concentration 2 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months
Supplier Images
anti-Autophagy Related 12 (ATG12) antibody Western blot analysis of anti-APG12 Monoclonal Antibody (ABIN387794) by APG12 recombinant protein. APG12(arrow) was detected using the purified Mab. Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue and lung carcinoma tissue reacted with APG12L Monoclonal Antibody (ABIN387794), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
anti-Autophagy Related 12 (ATG12) antibody (2) anti-Autophagy Related 12 (ATG12) antibody (Image 2)
Background publications Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry. ..." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).

Prigione, Cortopassi: "Mitochondrial DNA deletions and chloramphenicol treatment stimulate the autophagic transcript ATG12." in: Autophagy, Vol. 3, Issue 4, pp. 377-80, 2007 (PubMed).

Lee, Finkel: "Regulation of autophagy by the p300 acetyltransferase." in: The Journal of biological chemistry, Vol. 284, Issue 10, pp. 6322-8, 2009 (PubMed).

Kang, Kim, Oh et al.: "Frameshift mutations of autophagy-related genes ATG2B, ATG5, ATG9B and ATG12 in gastric and colorectal cancers with microsatellite instability." in: The Journal of pathology, Vol. 217, Issue 5, pp. 702-6, 2009 (PubMed).

Xia, Zhang, Chen et al.: "Control of basal autophagy by calpain1 mediated cleavage of ATG5." in: Autophagy, Vol. 6, Issue 1, pp. 61-6, 2010 (PubMed).

Validation Images
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