Autophagy Related 12 (ATG12) antibody

Antigen: Autophagy Related 12 (ATG12)

Clonality: Monoclonal (43CT73-3-5-5-4)

Host: Mouse

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387794

Additional Information

Alternative name: ATG12

Gene ID: 9140

UniProt: O94817

Immunogen: APG12L recombinant protein is used to produce this monoclonal antibody.

Isotype: IgG2b kappa

Clone: 43CT73-3-5-5-4

Description: Other names: APG12, APG12L, FBR93, HAPG12, APG12 autophagy 12-like, APG12-like, Apg12 (autophagy, yeast) homolog, autophagy-related protein 12

Characteristics: Purified Mouse Monoclonal Antibody (Mab)

Specificity: APG12L recombinant protein is used to produce this monoclonal antibody.

Molecular Weight: 15113 DA

Comments:

Background: Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:10000 Western blotting~~ 1:200~2000 Immunohistochemistry ~~ 1:50~200 Immunofluorescenc~~1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Apoptosis/Necrosis, Autophagy, Cell Structure

Restrictions: For Research Use only

Images

Western blot analysis of anti-APG12 Monoclonal Antibody (Cat.#ABIN387794) by APG12 recombinant protein. APG12(arrow) was detected using the purified Mab. Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue and lung carcinoma tissue reacted with APG12L Monoclonal Antibody (Cat.#ABIN387794), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry, clinical relevance has not been evaluated.

anti-Autophagy Related 12 (ATG12) antibody (Image 2)

Publications

Product: Ewing, Chu, Elisma et al.: "Large-scale mapping of human protein-protein interactions by mass spectrometry." in: Molecular systems biology, Vol. 3, pp. 89, 2007 (PubMed).

Prigione, Cortopassi: "Mitochondrial DNA deletions and chloramphenicol treatment stimulate the autophagic transcript ATG12." in: Autophagy, Vol. 3, Issue 4, pp. 377-80, 2007 (PubMed).

Lee, Finkel: "Regulation of autophagy by the p300 acetyltransferase." in: The Journal of biological chemistry, Vol. 284, Issue 10, pp. 6322-8, 2009 (PubMed).

Kang, Kim, Oh et al.: "Frameshift mutations of autophagy-related genes ATG2B, ATG5, ATG9B and ATG12 in gastric and colorectal cancers with microsatellite instability." in: The Journal of pathology, Vol. 217, Issue 5, pp. 702-6, 2009 (PubMed).

Xia, Zhang, Chen et al.: "Control of basal autophagy by calpain1 mediated cleavage of ATG5." in: Autophagy, Vol. 6, Issue 1, pp. 61-6, 2010 (PubMed).