Lysine (K)-Specific Demethylase 2A (KDM2A) (Center) antibody

Antigen: Lysine (K)-Specific Demethylase 2A (KDM2A)

Synonyms: FBL7, CXXC8, FBL11, FBXL11, JHDM1A, LILINA, FLJ00115, FLJ46431, KIAA1004, DKFZp434M1735, Fbl7, Cxxc8, Fbl11, Jhdm1, Fbxl11, Jhdm1a, lalina, AA589516, AW536790

Binding Site: Center

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387896

Additional Information

Alternative name: JHDM1a/FBXL11

Gene ID: 22992

UniProt: Q9Y2K7

Immunogen: This JHDM1a/FBXL11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 506~536 amino acids from the Center of human JHDM1a/FBXL11.

Isotype: Ig

Description: Other names: JmjC domain-containing histone demethylation protein 1A, [Histone-H3]-lysine-36 demethylase 1A, F-box/LRR-repeat protein 11, F-box and leucine-rich repeat protein 11, F-box protein FBL7, F-box protein Lilina, CXXC-type zinc finger protein 8, FBXL11

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This JHDM1a/FBXL11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 506~536 amino acids from the Center of human JHDM1a/FBXL11.

Molecular Weight: 132793 DA

Comments:

Background: JHDM1a/FBXL11 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box),which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbls class and, in addition to an F-box, contains at least 6 highly degenerated leucine-rich repeats.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100 Immunohistochemsitry ~~ 1:10~50

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Cell Structure

Restrictions: For Research Use only

Images

Formalin-fixed and paraffin-embedded human testis tissue reacted with JHDM1a/FBXL11 antibody (Center) (ABIN387896), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry. clinical relevance has not been evaluated.

Western blot analysis of JHDM1a/FBXL11 (arrow) using rabbit polyclonal JHDM1a/FBXL11 Antibody (Center)(ABIN387896).293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the JHDM1a/FBXL11 gene (Lane 2) (Origene Technologies).

JHDM1a/FBXL11 Antibody (Center) (ABIN387896) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the JHDM1a/FBXL11 antibody detected the JHDM1a/FBXL11 protein (arrow).

Publications

Product: Andersen, Lam, Leung et al.: "Nucleolar proteome dynamics." in: Nature, Vol. 433, Issue 7021, pp. 77-83, 2005 (PubMed).

Tsukada, Fang, Erdjument-Bromage et al.: "Histone demethylation by a family of JmjC domain-containing proteins." in: Nature, Vol. 439, Issue 7078, pp. 811-6, 2006 (PubMed).