USP21 antibody (N-Term)

Catalog No. ABIN387919

This Rabbit Polyclonal antibody specifically detects USP21 in WB and IHC (p). It exhibits reactivity toward Human and has been mentioned in 3+ publications.

Quick Overview for USP21 antibody (N-Term) (ABIN387919)

Target: USP21 (Ubiquitin Specific Peptidase 21 (USP21))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This USP21 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: RB2809

Product Details anti-USP21 Antibody

Binding Specificity: AA 1-30, N-Term

Predicted Reactivity: B, M, Rat

Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Immunogen: This USP21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human USP21.

Isotype: Ig Fraction

Application Details

Application Notes: WB: 1:2000. IHC-P: 1:50~100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.

Expiry Date: 6 months

References for anti-USP21 antibody (ABIN387919)

Jin, Liu, Chen, Liu, Jiang, Chu, Pan, Wang, Zhang, Li, Jiang, Ge, Xie, Wang: "The deubiquitinase USP21 maintains the stemness of mouse embryonic stem cells via stabilization of Nanog." in: Nature communications, Vol. 7, pp. 13594, (2018) (PubMed).

Tao, Chen, Song, Piccioni, Shi, Li: "Deubiquitination and stabilization of IL-33 by USP21." in: International journal of clinical and experimental pathology, Vol. 7, Issue 8, pp. 4930-7, (2014) (PubMed).

Zhang, Chen, Hou, Gao, Lin, Yang, Gao, Pan, Tao, Wen, Yao, Tsun, Shi, Li: "Identification of the E3 deubiquitinase ubiquitin-specific peptidase 21 (USP21) as a positive regulator of the transcription factor GATA3." in: The Journal of biological chemistry, Vol. 288, Issue 13, pp. 9373-82, (2013) (PubMed).

Target Details for USP21

Target: USP21 (Ubiquitin Specific Peptidase 21 (USP21))

Alternative Name: USP21

Background: USP21 is a ubiquitin-specific protease, an enzyme that removes ubiquitin from ubiquitinated proteins. The encoded protein belongs to the C19 peptidase family, also known as family 2 of ubiquitin carboxyl-terminal hydrolases. This protein has been reported to be capable of removing NEDD8 from NEDD8 conjugates.

Molecular Weight: 62656

Gene ID: 27005

NCBI Accession: NP_001014443, NP_036607

UniProt: Q9UK80