K(lysine) Acetyltransferase 2A (KAT2A) (N-Term) antibody

Antigen: K(lysine) Acetyltransferase 2A (KAT2A)

Synonyms: GCN5, hGCN5, GCN5L2, PCAF-b, MGC102791, Gcn5, Gcn5l2, mmGCN5, AW212720, 1110051E14Rik, im:7156024, gb:dq017634, KAT2A

Binding Site: N-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387930

Additional Information

Alternative name: GCN5

Gene ID: 2648

UniProt: Q8N1A2

Immunogen: This GCN5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72~102 amino acids from the N-term region of human GCN5.

Isotype: Ig

Description: Other names: GCN5 general control of amino-acid synthesis 5-like 2, Yeast, GCN5L2

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This GCN5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 72~102 amino acids from the N-term region of human GCN5.

Molecular Weight: 93836 DA

Comments:

Background: GCN5 functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Acetylation of histones gives a specific tag for epigenetic transcription activation. This protein has significant histone acetyltransferase activity with core histones, but not with nucleosome core particles.

Application Details

Application Notes: The suggested dilution is: ELISA ~~ 1:1,000 Western blotting~~ 1:50~100

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Transcription Factors, Chromatin

Restrictions: For Research Use only

Images

Western blot analysis of anti-GCN5 Antibody (N-term)(ABIN387930) in 293 cell line lysates (35ug/lane). GCN5(arrow) was detected using the purified Pab (1:60 dilution).

Publications

Product: Oishi, Kitagawa, Wada et al.: "An hGCN5/TRRAP histone acetyltransferase complex co-activates BRCA1 transactivation function through histone modification." in: The Journal of biological chemistry, Vol. 281, Issue 1, pp. 20-6, 2006 (PubMed).

Wiper-Bergeron, Salem, Tomlinson et al.: "Glucocorticoid-stimulated preadipocyte differentiation is mediated through acetylation of C/EBPbeta by GCN5." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 104, Issue 8, pp. 2703-8, 2007 (PubMed).