Histone Deacetylase 4 (HDAC4) (C-Term) antibody

Antigen: Histone Deacetylase 4 (HDAC4)

Synonyms: HD4, HDACA, HA6116, HDAC-A, KIAA0288, Hdac4, mHDA1, AI426555, mKIAA0600, AI047285, 4932408F19Rik, wu:fa96d08, wu:fc56f08, zgc:152701, CG1770, dHDAC4, DHDAC4, DmelCG1770, dmHDA405, GC1770, HDAC, HDAC4a, dana_GLEANR_19966, DanaGF18710, GF18710, HDAC4

Binding Site: C-Term

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Certificates: ISO 9001:2008

Catalog no.: ABIN387951

Additional Information

Alternative name: HDAC4

Gene ID: 9759

UniProt: P56524

Immunogen: This HDAC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human HDAC4.

Isotype: Ig

Description: Other names: Histone deacetylase 4, HD4, KIAA0288

Characteristics: Purified Rabbit Polyclonal Antibody (Pab)

Specificity: This HDAC4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human HDAC4.

Molecular Weight: 119069 DA

Comments:

Background: DNA is wrapped around histone proteins to form nucleosomes and chromatin fiber, a higher-order structure. Chromatin can become alternatively revealed to or concealed from transcription factors. Acetylation of lysine residues induces conformational changes in core histones by destabilizing nucleosomes and allowing transcription factors access to recognition elements in DNA. Deacetylation of histones by histone deacetylases (HDACs) reseals the chromosomal package, leading to a repression of transcription. HDAC4 does not bind DNA directly, but rather through MEF2C and MEF2D. Binding of the N terminus of HDAC4 to MEF2C represses MEF2C transcription activity. The catalytic domain of HDAC4 interacts with HDAC3 via the transcriptional corepressor NCOR2. Experimental conditions leading to the suppression of HDAC4 binding to NCOR2 and to HDAC3 result in loss of enzymatic activity associated with HDAC4, indicating regulation of transcription by bridging the enzymatically active NCOR2-HDAC3 complex and select transcription factors. HDAC4 and MITR contain calmodulin-binding domains that overlap with their MEF2 binding domains. Binding of calmodulin to HDAC4 leads to its dissociation from MEF2, relieving MEF2 from the transcriptional repression by HDAC4. Together, HDAC4, MITR, and CABIN1 constitute a family of calcium-sensitive transcriptional repressors of MEF2. In murine studies, HDAC4, which is expressed in prehypertrophic chondrocytes, interacts with and inhibits the activity of Runx2 in mice, a transcription factor necessary for chondrocyte hypertrophy, establishing HDAC4 as a central regulator of chondrocyte hypertrophy and skeletogenesis.

Synonyms: HD4, HDACA, HA6116, HDAC-A, KIAA0288, Hdac4, mHDA1, AI426555, mKIAA0600, AI047285, 4932408F19Rik, wu:fa96d08, wu:fc56f08, zgc:152701, CG1770, dHDAC4, DHDAC4, DmelCG1770, dmHDA405, GC1770, HDAC, HDAC4a, dana_GLEANR_19966, DanaGF18710, GF18710, HDAC4

Application Details

Application Notes: The suggested dilution will be: ELISA~~1:1,000 Western blotting~~1:100~500 Flow cytometry

Concentration: 0.25 mg/ml

Purification: Purified

Buffer: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Storage: Maintain refrigerated at 2-8 deg C for up to 6 months. For long term storage store at -20 deg C in small aliquots to prevent freeze-thaw cycles

Research Area: Signaling, Protein Modifications, Transcription Factors, Chromatin, Neurology, Cell Structure

Restrictions: For Research Use only

Images

The anti-HDAC4 Pab (ABIN387951) is used in Western blot to detect HDAC4 in mouse brain tissue lysate.

Publications

Product: Youn, Grozinger, Liu: "Calcium regulates transcriptional repression of myocyte enhancer factor 2 by histone deacetylase 4." in: The Journal of biological chemistry, Vol. 275, Issue 29, pp. 22563-7, 2000 (PubMed).

Kao, McKenna, Guenther et al.: "Histone deacetylase 4 interacts with 53BP1 to mediate the DNA damage response." in: The Journal of cell biology, Vol. 160, Issue 7, pp. 1017-27, 2003 (PubMed).